超声实时组织弹性成像评价大鼠肝纤维化分期

Evaluation of Rat Liver Fibrosis Using Real-time Tissue Ultrasonic Elastography

目的探讨超声实时组织弹性成像(RTE)评价大鼠肝纤维化分期的应用价值。材料与方法将80只雄性Wistar大鼠采用抽签法随机分为实验组(64只)和对照组(16只),实验组经腹腔注射CCl4橄榄油溶液制作肝纤维化模型,对照组经腹腔注射生理盐水。对大鼠肝脏进行RTE检查,获取弹性图像,测量硅胶垫与肝脏的应变比。选取与弹性成像感兴趣区一致的大鼠肝组织行病理检查,并对肝纤维化程度进行分期。结果各期肝纤维化应变比分别为S0期0.4211±0.1921、S1期0.4795±0.1109、S2期0.7370±0.1397、S3期0.7980±0.1001、S4期1.0279±0.2324,S0期与S1期比较无显著差异(P>0.05),与S2、S3、S4期比较有显著差异(P<0.001);S1期与S2、S3、S4期比较有显著差异(P<0.001);S2期与S3期比较无显著差异(P>0.05),与S4期比较有显著差异(P<0.001);S3期与S4期比较有显著差异(P<0.01)。应变比与病理分期呈显著正相关(r=0.799,P<0.05),随着肝纤维化程度加重,RTE应变比也随之增大。结论 RTE可以半定量评价大鼠肝纤维化,并进行肝纤维化分期,有望为临床肝纤维化研究提供初步参考。

Purpose To evaluate the value of real-time tissue elastography （RTE） for assessing rat liver fibrosis grading. Materials and Methods Eighty male Wistar rats were randomly divided into experimental group （n=64） and control group （n=16）. The experimental rats were intraperitoneally injected with CC14 olive oil solution. The control group was injected with saline. RTE was implemented to measure the strain ratio between reference silicon pad and rat liver. Histopathological examination was performed to grade liver cirrhosis. Results The strain ratios were 0.4211 ± 0.1921 for SO stage, 0.4795 ± 0.1109 for S1 stage, 0.7370±0.1397 for S2 stage, 0.7980±0.1001 for S3 stage, 1.0279±0.2324 for S4 stage, respectively, with significant difference between SO and S2, S3, S4 stages （P〈0.001）, and between S1 and S2, S3 and S4 stages （P〈O.O01）, and between S2 and S4 （P〈0.001）, S3 and S4 （P〈0.001）. Strain ratio and pathology classification were positively correlated （r=0.799, P〈0.05）. With the increased degree of liver fibrosis, RTE strain ratio was higher. Conclusion RTE cam semi-quantitatively evaluate liver fibrosis of rats, and further assess the liver fibrosis grading, which can provides initial reference for human liver fibrosis.