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细胞内氯离子通道蛋白2的定位与表达

The localization and expression of human chloride intracellular channel 2
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摘要 目的研究细胞内氯离子通道蛋白2(CLIC2)在细胞中的定位与表达。方法以含人CLIC2全长cDNA序列的菌液为模板,PCR法构建CLIC2真核表达载体,利用免疫荧光和Western blot法检测其在细胞株中的定位与表达。结果细胞免疫荧光结果显示CLIC2在细胞质、细胞核中均有分布;Western blot的结果证明其在细胞中能有效地表达。结论 CLIC2蛋白在细胞质和细胞核中均大量分布,为进一步了解CLIC2的功能奠定了基础。 Objective To investigate the location and expression of the chloride intracellular channel 2 （CLIC2） in human cells. Methods Bacteria liquid containing the full-length human cDNA sequence of CLIC2 was used as PCR template to construct the eukaryotic expression vector. Immunofluorescence and Western blot were adopted to detect the location and expression of CLIC2 in the cell strain. Results The results of immunofluorescence indicated that CLIC2 was located in both cytoplasm and cell nucleus. Besides, Western blot results showed that it can be effectively expressed in the cells. Conclusion CLIC2 is widely distributed and expressed in human cells, which lays foundation to further study the function of CLIC2.
作者 徐南 潘林鑫 耿慧武 李春雨 刘晓颖 范礼斌
机构地区 安徽医科大学生命科学院生物学教研室
出处 《安徽医科大学学报》 CAS 北大核心 2013年第5期455-457,共3页 Acta Universitatis Medicinalis Anhui
基金 安徽省教育厅自然科学重点科研项目(编号:KJ2010A187) 安徽省自然科学基金项目(编号:11040606M170 11040606M164)
关键词 细胞内氯离子通道蛋白2 免疫荧光 蛋白表达 CLIC2 immunofluorescence protein expression
分类号 R341 [医药卫生—基础医学] R394.2 [医药卫生—医学遗传学]
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参考文献11

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安徽医科大学学报
2013年 第5期

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