卵巢癌患者呼气中挥发性标志物的筛查

The screening of volatile markers in the breath of ovarian cancer patients

目的应用固相微萃取联合气相色谱/质谱技术筛查卵巢癌患者呼气中挥发性标志物。方法收集40例卵巢癌患者呼气样本(卵巢癌组),49例健康女性呼气样本(对照组),同时收集周围空气作为参考。利用固相微萃取联用气相色谱/质普检测筛查卵巢癌患者呼气中的挥发性有机化合物。通过t检验分析卵巢癌患者呼气中的特异性挥发性标志物。结果筛选出苯乙烯和壬醛两种特异性挥发性标志物。卵巢癌组苯乙烯的浓度为(268.854±96.660)ng/L,壬醛的浓度为(388.631±122.660)ng/L。两种物质的浓度在卵巢癌组和对照组之间差异有统计学意义(P<0.05)。结论苯乙烯和壬醛可能是卵巢癌患者呼气中潜在挥发性标志物。

Objective To screen the volatile organic compounds （VOCs） in ovarian cancer patients with solid phase micro-extract combined with the gas chromatography （GC）/mass spectrometry （MS）. Methods The breath samples were collected from 40 ovarian cancer patients and 49 healthy women as control. Besides, the ambient air was collected for reference. VOCs were extracted with SPME and analyzed by gas Chromatography （GC）/mass spectrometry （MS）. The student-t test was used for discriminating the VOCs and assessing the statistical significance. Results Styrene and nonanal were found as the specific VOCs for ovarian. The concentration range of the styrene was （268. 854 ±96. 660） ng/L,and the concentration range of nonanal was （388. 631 ± 122. 660） ng/L. The concentration of the two VOCs molecules were significantly different between ovarian cancer patients and healthy control （P 〈 0. 05）. Conclusion Styrene and nonanal may be considered as potential volatile markers for diagnosis of patients with ovarian cancer.