唑来膦酸对兔骨髓炎骨破坏进程的作用

Effect of zoledronic acid on bone destruction in rabbit' s osteomyelitis

[目的]了解唑来膦酸在新西兰大白兔胫骨骨髓炎发生过程中对骨破坏进程的作用。[方法]将新西兰大白兔35只分为三组,其中空白组5只,模型组(金黄色葡萄球菌于新西兰白兔右胫骨造骨髓炎模型)15只,唑来膦酸组(在模型组基础上联合使用唑来膦酸)15只,分别饲养2、4、6周后,进行X线片、血液WBC、CRP、骨钙素(BGP)、抗酒石酸酸性磷酸酶5b(TRACP-5b)等相关指标检测。[结果]X线片Norden评分唑来膦酸组均低于模型组,存在统计学差异(P<0.05)。模型组与唑来膦酸组WBC、CRP第2周时最高,之后逐渐下降,两组间始终无明显统计学差异,两组CRP与空白组相比存在统计学差异(P<0.01)。唑来膦酸组BGP第2、4周时较模型组下降,具统计学意义(P<0.05)。唑来膦酸组血清TRACP-5b水平均明显低于模型组(P<0.01),唑来膦酸组与空白组相比,自第4周出现统计学差异(P<0.01)。[结论]唑来膦酸在兔骨髓炎形成过程中能发挥一定的抑制破骨细胞活性作用,从而减缓骨破坏的进程,延缓骨髓炎的发展。

[ Objective ] To study the effect of zoledronic acid （ZA） on bone destruction in the New Zealand white rabbit tibia/osteomyelitis model. [ Methods] Thirty -five New Zealand white rabbits were divided into three groups： the blank group with 5 rabbits, the model group with 15 rabbits infected by staphylococcus aureus, the zoledronic acid group with 15 rabbits which the zoledronic acid was used on the basis of the model proup. The X -ray film, WBC, CRP, osteoealcin （BGP） and tar- trate resistant acid phosphatase （TRACP -5b） were detected at the 2na, 4th and 6th week. [Results] There was a statistically significant difference between the ZA group and model group on X - Norden osteomyelitis scores （ P 〈 0. 05 ） . WBC, CRP sig- nificantly increased at the 2~d week, and gradually decreased from the 4th week. There was not significant difference between the two groups. CRP of the two groups were always higher than in the blank group （P 〈0.01 ） . The serum BGP of the ZA group was lower than in the model group at the 2nd and 4th week （P 〈0. 05 ） . The serum TRACP -5b of the ZA group was significant- ly lower than in the model group （P 〈 0. 01 ） , and compared with the blank group, there was significant difference from the 4th week （P 〈 0.01 ） . [ Conclusion] Zoledronie acid can inhibit the osteoclast activity during the osteomyelitis formation process in the rabbit model. It can slow the bone destruction and delay the development of osteomyelitis.