摘要
以‘耐冬’子叶为外植体,对其愈伤组织途径再生植株进行了研究。以MS基本培养基,添加0.5mg·L-12,4-D+2.0mg·L-16-BA诱导愈伤组织,诱导率达94.5%。在添加0.1mg·L-1NAA和2.0mg·L-16-BA的继代培养基上经过2次转接继代培养后,将愈伤组织转入MS+0.5mg·L-1NAA+10.0mg·L-16-BA或者MS+0.5mg·L-1IBA+10.0mg·L-16-BA的分化培养基中,约45d可获得再生不定芽,分化率约34%。最后利用"滤纸桥生根法"获得了约33%的生根山茶植株,从而建立了‘耐冬’愈伤组织诱导及植株再生体系。
Callus induction and plantlet regeneration were studied from mature cotyledons of Camellia japoni- ca L. ＇Naidong＇. Up to 94.5% of the cotyledons cultured on MS medium with 0.5 mg.L1 2,4-D and 2.0 mg.L1 6-BA developed callus. The callus was then transferred to subculturing medium containing 0.1 mg.L1 NAA and 2.0 mg.L~ 6-BA. Adventitious buds were obtained after 45 days on the medium with 0.5 mg-L~ NAA＋10.0 mg.L-~ 6-BA or 0.5 mg.L-~ IBA＋10.0 mg.L16-BA, the differentiation rate was about 34%. About 33% plantes of C. japonica developed its fine root system by using ＂filter paper bridge rooting method＂. Therefore, callus in- duction and regeneration system were achieved successfully.
出处
《植物生理学报》
CAS
CSCD
北大核心
2013年第4期343-346,共4页
Plant Physiology Journal
基金
国家国际科技合作项目(2011DFA30490)
国家十二五科技支撑课题(2012BAD01B07)
关键词
山茶
'耐冬’
愈伤组织
不定芽分化
生根诱导
植株再生
Camellia japonica
＇Naidong＇
callus induction
adventitious buds differentiation
rooting induc- tion
plantlet regeneration
