Caspase-3表达强度评价新型医用高氮无镍奥氏体不锈钢的生物相容性

Evaluation of Biocompatibility of the Medical High-nitrogen Nickel-free Austenitic Stainless Steel by Detecting Expression of Caspase-3

目的:利用凋亡相关因子Caspase-3的表达强度分析新型医用高氮无镍奥氏体不锈钢的生物相容性。方法:设立实验组(新型医用高氮无镍奥氏体不锈钢组),对照组(317L不锈钢组、钴铬合金组、金合金组、空白组)。用5种液体分别培养L929细胞48h后,进行Hoechst33342/PI双染,荧光显微镜下观察活细胞、凋亡细胞、坏死细胞;用5种液分别培养L929细胞4h后收集细胞,提取蛋白,染色后用分光光度计测定A值,计算Caspase-3活化度。结果:各组细胞加样培养48h后Hoechst33342/PI双染,荧光显微镜观察实验各组均可见凋亡细胞;各组材料Caspase-3活化度由小到大依金合金组次为:空白对照组<金合金组<新型医用高氮无镍奥氏体不锈钢<钴铬合金<317L不锈钢。各组之间Caspase-3活化度有显著性差异(P<0.05)。结论:新型医用高氮无镍奥氏体不锈钢具有较佳的生物相容性,优于钴铬合金及317L不锈钢。

Objective： To evaluate the biocompatibility of BIOSSN4 by detecting caspase-3 activity.Methods： We set the experimental group（BIOSSN4） and control group（Au alloy,Co-Cr alloy,317L）,cultivated L929 cell by 4 kinds of liquor for 48h,observed of cell morphology by Hoechst33342/PI double staining,collected cells and extracted proteinum.After dyeing,we detected OD by spectra photometer and calculated the activity of Caspase-3.Results： Apptotic cells were seen under fluore scence microscope for each experimental grop after Hoechst33342/PI double staining.The activity of Caspase-3 from high to low was ：317L＆gt; Co-Cr alloy＆gt;BIOSS4＆gt; Au alloy＆gt;blank controller（P＆lt;0.05）.Conclusion： BIOSSN4 meets the requirement for clinical application,and the biocompatibility of BIOSSN4 is preliminarily safe for dental application.