摘要
目前体细胞克隆技术在畜牧业的许多领域都有着广泛的应用,但体细胞克隆效率低下却严重影响了该技术在畜牧业的发展。几乎所有的克隆动物都有胎盘肥大和表观遗传异常现象,这也是制约克隆效率的重要原因。综述了体细胞克隆的发展现状,阐述了体细胞克隆的胎盘肥大和重编程异常现象。并探讨了利用四倍体补偿法改善克隆胎盘肥大和通过对表观遗传基因的调控提高克隆效率的新方法。
Somatic cell nuclear transfer （SCNT）is widely used in animal hunsbandry. However, the low efficiency restricted its application severely. Almost all the cloned animals have the hypertrophic placenta and aberrantly epigenetic inheritance which also result into the low efficiency of the cloning technology. The current progresses of SCNT were summarized and a new method, tetraploid compensation, to improve the placenta hypertrophy and the efficiency cloning by epigenetic modification were disccussed.
出处
《中国生物工程杂志》
CAS
CSCD
北大核心
2013年第4期136-142,共7页
China Biotechnology
关键词
体细胞克隆
胎盘肥大
滋养层缺陷
四倍体补偿法
表观遗传修饰
Somatic cell nuclear transfer
Placenta hypertrophy
Trophoblast defect
Tetraploid compensation method
Epigenetic modification
