胃癌患者外周血CD4＋CD25high CD127low/-调节性T细胞表达临床意义分析

Detection and clinical analysis of CD4^+ CD25^(high)CD127^(low/-)regulatory T cells in peripheral blood of patients with gastric cancer

目的:研究CD4+CD25highCD127low/-调节性T细胞(Treg)在胃癌患者外周血中的表达水平,探讨其在肿瘤发病机制及治疗中的作用。方法:采用流式细胞术检测法检测90例胃癌患者(35例为早中期胃癌,55例为晚期胃癌)和30名健康体检者外周血中CD4+CD25highCD127low/-Treg的表达水平。结果:90例胃癌患者外周血CD4+T细胞中CD4+CD25highCD127low/-Treg含量为(11.60±5.99)%,高于健康体检者(5.19±1.72)%,t=5.610,P=0.007;Ⅲ+Ⅳ期患者外周血CD4+T细胞中CD4+CD25high CD127low/-Treg含量为(12.55±6.59)%,明显高于Ⅰ+Ⅱ期患者(10.39±4.68)%(t=4.113,P=0.04)和健康体检者(5.19±1.72)%(t=5.923,P=0.001)。手术后胃癌患者CD4+CD25highCD127low/-Treg水平为(6.12±2.13)%,明显低于手术前(11.25±5.63)%,t=5.237,P=0.04。结论:胃癌患者外周血CD4+CD25highCD127low/-Treg数量增多且与病程分期相关,手术后Treg数量明显减少,Treg可能参与了胃癌的发生发展。

OBJECTIVE：To research the level of CD4＋CD25high CD127low/- regulatory T cells （Treg） in the patients with gastric cancer and investigate the role in pathogenesis and treatment of cancer. METHODS： The peripheral blood from 30 healthy subjects and 90 gastric cancer patients were used to detect the proportion of CD4＋CD25high CD127low/- Treg with the multi-color detection and multi-parameter flow cytometry. RESULTS.. Compared with healthy group [（5.19±1.72） %] ,the proportion of CD4＋CD25high CD127low/- Treg in the peripheral blood of cancer patients[（11.60±5.99）%] showed a significant increase （t= 5. 610, P=0. 007）. With the progress of the disease, the proportion of CD4＋CD25high CD127low/- Treg in patients of stage III - IV[（12.55±6.59）% -] was significantly higher than that in patients of stage I ＋ II [（10.39±4.68）% ,t=4. 113,P=0.04] and healthy individuals[（5.19±1.72）% ,t=5. 923,P=0. 001）]. The proportion of CD4＋CD25high CD127low/- Treg was significantly lower in postoperative patients[（6. 12 ± 2.13）%] than that in preoperative patients[（11.25 ± 5.63） %, t= 5. 237, P： 0.04]. CONCLUSIONS： The proportion of CD4＋CD25high CD127low/- Treg in the peripheral blood of cancer patients is related to the tumor stage,and its number in postoperative patients showed a significant reduction. CD4＋CD25high CD127low/- Treg may be involved in the development of gastric carcinogenesis and may become an important molecular target for detection of the immune status of patients with gastric cancer.