胃肠道恶性肿瘤髓过氧化物酶基因多态性及其活力的相关性研究

A Study on Polymorphism of Myeloperoxidase Gene of Gastrointestinal Cancer and Its Correlation with the Activity

目的:了解胃肠道肿瘤患者血液白细胞髓过氧化物酶(myeloperoxidase MPO)基因的多态性及血清酶活力,分析其与胃肠道肿瘤发生的关系。方法:100例胃肠道恶性肿瘤为病例组,100例健康体检者为对照组,采集两组人群静脉血,采用等位基因特异性引物PCR(ASP-PCR)方法检测MPO-463 G/A位点的多态性,紫外分光光度法检测MPO活力。结果:恶性肿瘤组MPO-463G/A位点GG、GA和AA基因型分布频率为70%(70/100)、20%(20/100)和10%(10/100),对照组为58%(58/100)、36%(36/100)和6%(6/100),两组比较差异有统计学意义(χ2=6.03,P<0.05);肿瘤组与对照组MPO酶活力比较差异有统计学意义(F=0.76,P<0.05);MPO-463G/A位点基因GA+AA与GG基因型个体MPO酶活力比较,差异有统计学意义(F值分别为0815,0.7625,P<0.05)。结论:MPO基因463G/A位点多态性与胃肠道恶性肿瘤的发病风险有关;不同MPO基因型导致酶活力改变,此改变可能受MPO-463G/A多态性的影响。

Objective： To understand myeloperoxidase （MPO） gene polymorphism and its activity in patients with gastrointestinal cancer, and to analyze the relationship between MPO and the occurrence of gastrointestinal tumors. Methods： One hundred cases with gastrointestinal tumor and 100 healthy persons served as cancer group and control group respectively. Venous blood was collected. Allele-specific primer PCR （ASP-PCR） method was used for detection of polymorphism of MPO-463G/A locus. MPO activity was examined with UV speetrophotometry. Results： The frequencies of GG, GA and AA genotypes of MP0-463 were 70% （70/100）, 20% （20/100） and 10% （10/!00） in cancer group and were 58% （58/100）, 36% （36/100） and 6% （6/100） in control group respectively. The difference between the two groups was statistically significant （x^2 = 6.03, P 〈 0.05 ）. The difference of MPO activities between cancer group and control group was statistically significant （ F = 0. 76, P 〈 0.05 ）. The differences of MPO activities between genotypes GA ＋ AA and GG were statistically significant （F = 0815, 0. 7625, P 〈 0.05 ）. Conclusions： MPO463G/A gene polymorphism relates to gastrointestinal cancer and different MPO genotype may lead to activity change which may be influenced by MPO463G/A gene polymorphism.