8株猪繁殖与呼吸综合征病毒ORF5基因的变异分析

Genetic Variation Analysis of ORF5 Gene of 8 Porcine Reproductive and Respiratory Syndrome Virus Isolates

本研究拟初步了解中原地区近期猪繁殖与呼吸综合征病毒(PRRSV)的变异情况。对2011—2012年间从河南及周边省份发病猪场分离的8株PRRSV毒株Nsp2基因变异区进行RT-PCR扩增,同时对主要结构蛋白GP5基因进行克隆测序并与GenBank中登录的中国历年来流行的PRRSV代表毒株的GP5蛋白序列进行遗传进化分析,对主要氨基酸基序进行比对分析。结果表明,2011—2012年在中原地区分离的8株PRRSV分离株均为Nsp2缺失变异株,GP5基因与2006年中国高致病PRRSV代表毒株JXA1同源性较高。

Real time monitoring information was analyzed to understand the genetic and pathogen- ic evolution of porcine reproductive and respiratory syndrome virus （PRRSV） in Central China during 2011-2012. RT-PCR was conducted to identify the discontinuous 29-amino acid deletion in the Nsp2 gene, which is the characteristic of highly-pathogenetic PRRSV （HP-PRRSV）. The GP5 gene of 8 PRRSV isolates were cloned, sequenced and subsequently compared with that of 62 typical isolates deposited in GenBank over the years. The results showed that all the 8 isolates collected in Central China during 2011-2012 were closely related to the HP-PRRSV emerged in Southern China in 2006.