摘要
为明确中国安徽省鹅细小病毒(Goose parvovirus,GPV)Y株(番鸭源)和E株(鹅源)的全基因组信息,并进而为研究安徽省GPV的遗传演化、抗原性差异和致病性等提供参考依据。笔者应用PCR方法获得GPV Y株和E株的全基因组核苷酸序列,并与GenBank收录的全部GPV与番鸭细小病毒(Muscovy duck parvovirus,MD-PV)FM株进行核苷酸比较。结果表明2株GPV的全基因组核苷酸序列均与中国台湾82-0321株和06-0329株的相似性最高,Y株全基因组为5 106bp,E株为5 125bp,E株与Y株相比稍有不同,即在ITR区缺失1个碱基,在VP3阅读框之后,Poly A信号之前的4646—4668nt处多出1段22bp的核苷酸;通过比较各国GPV的VP3基因,发现GPV主要分属欧洲株(Ⅰ亚群)和中国株(Ⅱ亚群),中国大陆大多数GPV株属于Ⅱb亚群,但Y株和E株属于中国台湾Ⅱa亚群,再次表明安徽E株和Y株与中国台湾各毒株较为接近;以标准强毒B株为参考,通过分析VP区糖基化位点的差异发现Y株和E株均缺失703—705NRT糖基化位点,推测结构蛋白糖基化位点的改变有可能影响病毒的毒力,甚至使病毒形成免疫逃逸。
The aim of this study was to provide reference for genetic evolution, antigenic specifici- ty and pathogenicity of goose parvovirus (GPV) in Anhui Province of China. The complete ge- nomic sequences of two GPV strains isolated from Muscovy ducks (GPV Y stain) and geese (GPV E stain) in Anhui Province were obtained by polymerase chain reaction method and then aligned with the sequences of GPV and Muscovy duck parvovirus (MDPV) published in GenBank using the neighbor-joining method. The results showed that the genome of the GPV Y stain (5 106 bp) was shorter than that of the GPV E stain (5 125 bp), which was attributed to a 1-bp deletion in the ITR region and a 22-bp addition in the region (4646-4668 nt) between the end of VP30RF and the beginning of its poly(A) tail in the GPV E stain. The phylogenetic analyses re- vealed that these two strains along with those from Taiwan Province of China belonged to the GPV subgroup IIa, while most of other GPV stains isolated in China's Mainland were clustered in the GPV subgroup IIb. This result further confirmed the high homology of the two isolates to those from Taiwan Province. Compared with the standard virulent GPV B strain, the two isolates missed the deduced 703-705NRT glycosylation site in VP region, which may affect the viral viru- lence and even cause immune escape.
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2013年第4期602-609,共8页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
关键词
鹅细小病毒
全基因
VP基因
分子特征
goose parvovirus
complete genome
VP gene
molecular characterization