摘要
为证实传染性法氏囊病毒(IBDV)感染SPF雏鸡后,其免疫器官细胞凋亡与bcl-2mRNA动态表达的关系,应用TUNEL和实时荧光定量PCR方法分别对雏鸡免疫器官细胞凋亡数量和bcl-2mRNA表达的动态变化进行了检测,结果发现,IBDV感染雏鸡后3～7d,法氏囊、胸腺和脾脏中凋亡细胞数量显著或极显著高于相应对照雏鸡(P<0.01或P<0.05);同时在上述3种免疫器官中bcl-2mRNA的表达均不同程度增加,并于病毒感染后3～7d差异显著或极显著(P<0.01或P<0.05)。表明IBDV可增加感染雏鸡免疫器官的细胞凋亡数量和bcl-2mR-NA的表达。
Apoptosis and expression of bcl-2 mRNA in the immune organs of chicks infected with infectious bursal disease virus （IBDV） were investigated. The number of apoptosis cells was as- sessed by TUNEL method. The expression of bcl-2 mRNA was investigated by Real-time PCR. The results showed that the apoptosis cells in bursa of Fabricius, thymus and spleen of chicks in- fected with IBDV from the third to seventh day post infection are significant or extremely signifi- cant higher than that of normal SPF chicks （P〈0.01 or P〈0.05）. At the same time, the expres- sion of bcl-2 mRNA significantly increased in the immune organs of SPF chicks infected with IB- DV from the third to seventh day post infection compared with those of normal chicks （P〈0.01 or P〈0.05）. These study indicates that IBDV can improve the number of apoptosis and the ex- pression of bcl-2 mRNA in immune organs of chicks infected with IBDV.
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2013年第4期617-621,共5页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
国家自然基金青年基金(30901062)
