基因C型鸭甲肝病毒实验感染雏鸭的组织病理学及病毒分布

Pathology and Virus Distribution of Duck Hepatitis A Virus Genotype C in Experimentally Infected Ducklings

旨在研究基因C型鸭甲肝病毒(DHAV-C)感染导致雏鸭的组织病理学变化和病毒的组织分布,为该病毒的致病机理研究奠定基础。本研究以基因C型鸭甲肝病毒感染SPF雏鸭,感染后不同时间点采取雏鸭10个不同组织器官制备组织切片,通过HE染色和免疫酶组织化学染色对感染雏鸭的组织病理学变化及病毒抗原免疫组化定位进行观察。结果表明:DHAV-C感染导致雏鸭的心脏、肝脏、脾脏、肺脏、肾脏、小肠、胸腺、胰脏、大脑和法氏囊共10个组织器官均发生了不同程度的病变,同时在这些器官中均有病毒抗原检出,在10个检测组织器官中,以肝脏、脾脏、肾脏、法氏囊、胸腺和胰脏等器官的组织病变和抗原检出最为明显,病变的严重程度与病毒抗原检出量成正相关。DHAV-C广泛分布到受检组织器官中,它导致的组织病理学变化严重程度与病毒抗原的检出量成正相关,推测DHAV-C入侵导致肝、肾和免疫器官的损害,是其急性感染发病的根本原因。

This experiment was conducted to study the histopathological changes and virus antigen distribution caused by Duck hepatitis A virus genotype C （DHAV-C） in Specific pathogen free （SPF） ducklings in order to partially elucidate the pathogenesis of the virus. SPF ducklings were experimentally infected with DHAV-C and 10 sorts of organs from the infected ducklings were fixed in 4% paraformaldehyde at different time points post infection （PI）. The prepared sections from 10 organs of DHAV-C infected ducklings at different time points PI, were stained with hae-matoxylin and eosin （HE）, as well as Immunohistochemical （IHC） staining and were observed under a light microscope. The results showed that the 10 examined organs, including heart, liv- er, spleen, lung, kidney, intestine, thymus, bursa of Fabricus （BF）, pancreas and cerebrum, all had pathological changes and virus antigen distributions. The severe pathological changes and more virus antigens were found in liver, spleen, kidney, bursa of Fabricus （BF）, thymus and pancreas. Where there were more virus antigen stains, there were more severe histopathological changes. These results indicated DHAV-C causes wide damages to different duckling organs. The virus invasion to liver, kidney and immune organs thymus, bursa of Fabricus （BF） and spleen is the foundmental reason for its acute infection.