氯胺酮对树突细胞体外分化和成熟的影响

Effect of Ketamine on Differentiation and Maturation of Dendritic Cells in Vitro

【目的】观察氯胺酮对树突细胞体外分化和成熟的影响。【方法】取6～8周C57BL/6野生型老鼠(雌雄不限)的骨髓细胞,在FMS样酪氨酸激酶3(Flt3)配基的作用下诱导分化为树突细胞各种亚型。在培养体系中加入50、100、200μmol/L的氯胺酮,观察氯胺酮对树突细胞亚型分化比例的影响。同时为了观察氯胺酮对树突细胞成熟的影响,利用脂多糖刺激树突细胞成熟并检测氯胺酮对树突细胞表面成熟标记分子表达的影响。【结果】200μmol/L氯胺酮不明显影响pDc和cDc的分化比例,在100和200μmol/L可以使eCD8+cDc亚群分别降低(20±2.9)%和(25±4.1)%(P<0.05),而eCD8-cDc亚群则分别升高(25±4.3)%及(39±5.7)%(P<0.05)。200μmol/L氯胺酮不影响脂多糖介导的树突细胞成熟分子的表达。【结论】高浓度的氯胺酮可能通过影响树突细胞亚群分化而影响机体的免疫,但不影响树突细胞体外成熟标志的表达。

[Objective] To investigate the effect of ketamine on the differentiation and maturation of dendritic cells in vitro. [Methods] Bone marrow from 6-8 weeks of C57BL/6 wild-type mice was collected and differentiated into dendritic cells with the support of FMS-like tyrosine kinase 3 ligand （Flt3 ligand）. Dendritic cells were treated with different concentrations of ketamine （50 μmol/L, 100μmol/L, and 200 μmol/L） to detect the differentiation of different subpopulation. LPS 100 ng/mL was also used to stimulate the maturation of dendritic cell. Thereafter, we detected the expression of mature molecule on dendritic cell surface. [Result] 200 μmol/L of ketamine did not show obvious influence on pDc and cDc differentiation ratio. However, in cDc subsets, eCD8＋Dc subsets were reduced by （20 ±2.9）% and （25± 4.1）% （P 〈 0.05）; eCD8- Dc subsets were increased by （25 ± 4.3）% and （39 ± 5.7）% （P 〈 0.05） after treatment of 100 μmol/L and 200 μmol/L of ketamine, respectively. 200μmol/L of ketamine did not affect LPS-mediated mature molecule expression on dendritic cells. [ Conclusion ] High concentrations of ketamine may influence the differentiation of dendritic cell subsets and thus affect the body＇s immune function. However, high concentrations of ketamine did not affect the expression of maturation signs of dendritic cell in vitro.