比格犬卵巢子宫内雌激素受体ERα、ERβ的免疫组织化学定位

Immunohistochemical localization of estrogen receptor-α and-β in the ovary and uterus of Beagle dogs

目的研究雌激素受体α,β在比格犬卵巢及子宫内的定位。方法采用免疫组化SP法DAB显色结合BCIP/NBT及AEC显色检测ERα、ERβ在比格犬子宫及卵巢内的表达。结果比格犬ERα主要表达于卵泡颗粒细胞、卵巢间质腺腺上皮细胞及子宫内膜腺体腺上皮细胞胞核内,胞质内仅有少量表达,而在卵泡膜内膜的间质细胞,腺体周围的基质细胞及小动脉血管内皮细胞和平滑肌细胞、小静脉内皮细胞的胞核内有少量表达。而ERβ则以相同的组织特异性主要表达于上述组织细胞的胞质内,在胞核内有微弱表达。ERα表达于膜黄体细胞的胞核内,而在黄体颗粒细胞胞核与胞质内均有表达。而ERβ则仍特异表达于不同生理阶段黄体细胞的胞质内。BCIP/NBT与AEC双染未见ERα、ERβ在子宫内有明显的共表达现象。结论比格犬ERα、ERβ在子宫与卵巢组织内定位不同,ERα主要定位于胞核,在胞质内有微弱表达,而ERβ主要定位于胞质,在胞核内有零星表达。

Objective To examine immunohistochemically the expression of estrogen receptor-α （ERa） and -β （ER（β） in different types of cells in the ovary and uterus of Beagle dogs. Methods Six multiparous female Beagle dogs were used in this study. S-P immunohistochcmistry was used to detect the expression of ERa and ERα in the dog uterus and ovary with DAB, BCIP/NBT and AEC staining. Results ERa was mainly expressed in the nuclei of granulosa cells, ovar- ian interstitial glandular epithelial cells and endometrial glandular epithelial cells （ along with weaker staining in the cyto- sol）. It was also sporadically expressed in the nuclei of the theca cells, peri-glandular stromal cells, arteriolar endothelial cells, artery smooth muscle cells and small vein endothelial cells. On the contrary, ERβ was mainly expressed in the cyto- plasm of all of the above cells （along with sporadic staining in the cell nuclei）. In addition, in the corpus luteum at differ- ent physiological periods, ERa was immunohistochemically positive in the nuclei of theca cells, and also in the nuclei and cytoplasm of luteinizing granulosa cells, while ERβ was still positive expressed in the cytoplasm of luteal cells. Coexpres- sion of ERa and ERβ in the dog uterus was not detected with BCIP/NBT and AEC double staining. Conclusions The re- suits of our study show that the expressions of ERa and ERβ in the uterus and ovary of Beagle dogs are different. EBα mainly located in the nuclei of different cell types（ along with Weaker staining in the cytosol）, whereas ERβ is mainly lo- calized in the cytoplasm of different cell types （ along with sporadic staining in the nuclei）.