超声在评价大鼠酒精性脂肪肝模型中的应用

Application of ultrasonography in the estimation of rat models of alcoholic fatty liver

目的利用超声技术来评价大鼠酒精性脂肪肝动物模型。方法选取40只SD大鼠随机分为两组(n=20只)。模型组按每周测定的体重早晚各1次乙醇灌胃(10 g/kg),第1周浓度为40%,第2、3周分别为45%和50%,第4周为55%灌胃直至12周;对照组给予等体积的生理盐水灌胃。造模于第4、8和12周时对两组大鼠进行超声监测,并从两组中各随机抽取3只大鼠进行肝脏病理学分析,与超声监测结果进行对比分析。结果超声与病理检查结果均提示酒精性脂肪肝造模成功,超声可以监测模型组大鼠肝脏脂肪病变从轻到重的渐变过程以及对照组大鼠无脂肪病变过程。这与肝组织的病理学诊断结果具有一致性。结论超声检测技术可以较好地进行活体评价大鼠酒精性脂肪肝动物模型。

Objective To evaluate the feasibility of ultrasonography in the estimation of alcoholic fatty liver in rat models. Methods Forty healthy male SD rats were randomly divided into two groups （n = 20 ）. According to the body weight measured weekly, the rats in the model group were garaged with ethanol （ 10 g/kg b. w. , twice per day）. The ethanol concentration was 40% at the first week, 45% at the second and 50% at the third week. Since the forth week, the ethanol concentration was increased to 55% until the twelfth week. Rats in the normal control group were gavaged with physiological saline. At the end of weeks 4, 8 and 12, the rats in the two groups were monitored with ultrasonography, and 3 rats from each group were randomly selected and sacrificed for pathological examined. Finally, the results of ultrasonography and pathology were compared and analyzed. Results Ultrasonography and pathological examination showed that alcoholic fatty liver models were successfully established, and ultrasonography can be used for monitoring the gradual development process of rat liver fatty lesions （from mild to severe） in the mode] rats, and the control rats showed no fatty lesions. The results of ultrasonography were consistent with the pathological examination results. Conclusion Ultrasonography can well be used for evaluation of alcoholic fatty liver in living rat models.