摘要
将茶多酚(TP)与聚乳酸(PLA)共混,采用静电纺丝方法制备PLA/TP复合纳米纤维膜。通过红外光谱(FT-IR)测试、抑菌圈法及流式细胞仪(FACS)对复合纳米纤维膜的成分、抗菌性能及抗菌机理进行研究。FT-IR测试结果验证了PLA/TP复合纳米纤维膜中通过价键的结合使两者复合在一起。抗菌测试结果显示:随着TP含量增加,复合纳米膜抗菌性能提高。对大肠杆菌和金黄色葡萄球菌的抑菌圈宽度分别从3.67cm和3.71cm增加到5.17cm和5.67cm。FACS结果表明:PLA/TP复合纳米纤维膜对比纯PLA纳米纤维膜对大肠杆菌和金黄色葡萄球菌的PI染色率明显增加,分别从0.78%和1.45%增加到6.47%和9.26%,揭示了PLA/TP复合纳米纤维膜能够破坏菌体细胞膜的完整性,最终导致其死亡。
Electrospinning was conducted of the blend of Tea polypheno （TP） and Polylactic acid （PLA） to produce PLA/TP composite nanofiber films with high antibacterial function. The composition, antibacterial performance and mechanism of this composite nanofiber films were investigated using infrared spectroscopy （FT-IR）, inhibition zone method and Fluorescen Activated Sorter （FACS）. IR spectra showed that the TP and PLA are chemically bonded to become the PLA/TP composite nan,：fiber films. The antibacterial experiments suggested that the antibacterial perfoemence increases as TP content increnses. The width of inhibition zone against Escherichia coli （Staphylococcus aureus） is increased from 3.67 （3.71cm） to 5.17 （5. 67cm）. h is implied by FACS that positive rate of PLA/TP composite nanofibrous films was greater than pure PLA nanofibrous films, which increased from 0. 78% （1.45%） to 6.47% （9. 26%） against S. aureus （E. coli）, The PLA/TP composite nanofiber films were revealed to destroy the integrity of cell membrane thus leading to the death of bacterium.
出处
《材料科学与工程学报》
CAS
CSCD
北大核心
2013年第2期181-185,174,共6页
Journal of Materials Science and Engineering
基金
教育部长江学者和创新团队发展计划资助项目(IRT1135)
关键词
茶多酚
聚乳酸
纳米纤维膜
抗菌机理
tea polypheno
polylactic acid
nanofiber film
antibacterial mechanism
