摘要
目的筛选二线抗结核药物对氨基水杨酸（para-aminosalicylic acid，PAS）耐药相关基因。方法构建H37Rv转座子文库，筛选对氨基水杨酸耐药克隆，随机引物PCR扩增转座子插入突变基因。在PAS耐药菌株中鉴定耐药相关基因的突变情况。结果构建了较完整的H37Rv转座子文库，并筛选出201个PAS耐药克隆，经测序找到4个可能的PAS耐药相关基因。papA1基因检测到一个无义突变。结论酰基转移酶基因papA1可能为PAS耐药相关基因。
Objective To screen the PAS （para-aminosalicylic acid） resistant-related genes. Method We constructed the H37Rv transposoninsertion library, screened the PAS resistant clone, amplified the mutant gene with transposon-insertion through random primer PCR. Furthermore, the mutants of the PAS resistant-related genes were examined in the PAS clinical resistant strain. Result The H37Rv transposon-insertion library was constructed successfully, and 4 possible PAS resistant-related genes were identified. A nonsense mutation of papAl was found in the PAS clinical resistant strain. Conclusion papA1 may be the PAS resistant-related gene.
出处
《结核病与胸部肿瘤》
2012年第3期162-165,共4页
Tuberculosis and Thoracic Tumor
基金
基金项目：国家传染病重大专项结核病诊断分子标识研究2012ZX10003002-003
北京市卫生系统高层次卫生技术人才培养计划2009-3-51
关键词
分枝杆菌
结核
耐药
对氨基水杨酸
基因
Mycobacterium tuberculosis
Drug resistance
Para-aminosalicylic acid
Gene
