宁波港压载水的细菌多样性研究

Study on genetic diversity of bacteria in ballast water from Ningbo port

为揭示宁波港压载水的微生物群落结构在人类活动影响下的分布差异及对环境因子变化的响应趋势。该课题从压载水中分离细菌,通过微生物自动鉴定系统(Sherlock MIS)、分子生物学16S rRNA基因的PCR扩增及RFLP技术,对宁波港压载水的细菌结构特征进行比对和分析,并通过16S rRNA基因文库解析研究压载水中微生物群落多样性。结果表明印度洋水样中存在假交替单胞菌属(Pseudoalteromonas sp.)、交替单胞菌属(Alteromonas sp.)、海杆菌属(Marinobacter sp.)和玫瑰杆菌属(Roseobacter sp.)、肠杆菌属(Enterobacter sp.);新加坡水样中存在人苍白杆菌属(Ochrobactrum sp.)、甲基杆菌属(Methylobacterium sp.)、鞘鞍醇单胞菌(Sphingomonas sp.)、嗜冷杆菌属(Psychrobacter sp.)、假单胞菌(Pseudomonas sp.)、伯克霍尔德氏菌(Burkholderia sp.)和葡萄球菌属(Staphylococcus sp.)假单胞菌属(Pseudomonas sp.);而美洲水样中存在弧菌属(Vibrio sp.)、军团菌属(Legionella sp.)、莫拉氏菌属(Moraxella sp.)和不动杆菌属(Acinetobacter sp.)。压载水中含有大量的潜在致病菌。

We investigated the impacts of anthropogenic activities on structure and composition of bacterial communities and evaluated how bacterial communities responded to environmental gradients at Ningbo port.Bacteria in the ballast water from Ningbo port were isolated,and the bacterio plankton diversity was studied by using the Sherlock Microbial Identification System,16S rRNA gene PCR amplification,and restriction fragment length polymorphism.Meanwhile,16S rRNA clone library was constructed to reveal the composition and structure of bacterial communities in the most of ballast water.The results showed that there were Alteromonas sp.,Pseudoalteromonas sp.,Marinobacter sp.and Roseobacter sp.in Indian Ocean,Ochrobactrum sp.,Methylobacterium sp.,Pseudomonas sp.,Psychrobacter sp.,Pseudomonas sp.,Burkholderia sp.and Staphylococcus sp.in Singapore ballast water,Vibrio sp.,Legionella sp.,Moraxella sp.and Acinetobacter sp.in American ballast water.Ballast water contains a large number of potential pathogens.