IL-6在慢性阻塞性肺疾病合并糖尿病大鼠肺组织中水平变化的实验研究

Research on the Concentration Variation of IL-6 in the Lung Tissue of Chronic Obstructive Pulmonary Disease Rats Combined with Diabetes

目的探讨白细胞介素6(IL-6)在慢性阻塞性肺疾病(COPD)大鼠、糖尿病大鼠、慢性阻塞性肺疾病合并糖尿病大鼠肺组织中的浓度水平变化及其意义。方法清洁级雄性SD大鼠80只,随机分为4组即COPD组、糖尿病组、COPD合并糖尿病组和正常对照组,各组均为20只。采用每日熏香烟法制造COPD组模型,饲以高脂饲料并注射小剂量链脲佐菌素法制造糖尿病组模型,合并上述方法制造COPD合并糖尿病组模型。造模后提取各大鼠肺组织,通过病理形态学检测证明造模成功,并采用免疫组织化学方法检测4组大鼠肺组织中IL-6的浓度水平。结果 1.COPD组和COPD合并糖尿病组大鼠支气管鳞状上皮化生、呼吸道壁纤维化及肺泡壁的破坏和纤维化上皮明显增生增厚,壁有大量淋巴细胞、巨噬细胞为主的炎细胞浸润,较多中性粒细胞浸润,肺泡腔扩大,肺泡壁断裂融合形成肺泡气肿,COPD合并糖尿病组较COPD组、糖尿病组病理表现更为显著。2.免疫组化结果显示:正常组IL-6浓度为(4.27±0.89)%,COPD组IL-6浓度为(13.13±1..28)%,糖尿病组IL-6浓度为(8.26±0.92)%,COPD合并糖尿病组IL-6浓度为(20.86±2.73)%。与正常组比较,COPD组、糖尿病组和COPD合并糖尿病组IL-6水平均升高(P<0.01);与COPD组比较,糖尿病组IL-6降低(P<0.01),COPD合并糖尿病组IL-6升高(P<0.01);与糖尿病组比较,COPD合并糖尿病组IL-6升高(P<0.01)。结论大鼠肺组织中IL-6水平的增高与COPD及高血糖的炎性反应有叠加作用。

Objective In order to research the concentration variation and effect of IL-6 on the lung tissue of Chronic Obstructive Pulmonary Disease rats（COPD）, diabetic rats as well as Chronic Obstructive Pulmonary Disease combine with diabetic rats. Methods Participants were 80 male SD rats in clean grade they were divided into 4 groups randomly including chronic obstructive pulmonary disease, combine with diabetes mellitus and normal control group. diabetes mellitus, chronic obstructive pulmonary disease each group has 20 rats. COPD rats are from smoking of cigarette everyday. Diabetes mellitus rats are by abdominal cavity Injection with streptozotocin （STZ） and high-fat feed. Combining the two methods obtain the chronic obstructive pulmonary disease combine with diabetes mellitus rats. After the models have been established, the rat lung tissues were extracted. The concentration variation of IL- 6 in the four groups rat lung tissue was detected by immunohistochemistry. Results 1. The rats of chronic obstructive pulmonary disease and chronic obstructive pulmonary disease combine with diabetes mellitus have changes as follows： the bronchus of cervical mucosa, respiratory tract wall tube fibrosis as well as alveolar wall break and fibrosis, epidermis hyperplasia and incrassation, mainly soakage of inflammatory cell of collections of lymphocytes and macrophage on bronchus wall, more soakage of neutrophilic granulocyte, alveolar space large, alveolar walls part forming alveolar ectasia. The group of chronic obstructive pulmonary disease and diabetes mellitus is more marked than the others. 2. Immunohistochemical results show that ： The positive change of IL-6 in normal group is（4. 275±0. 89）% ; the positive change of IL-6 in COPD group is（13. 13±1.28） % ; the positive change of IL-6 in diabetes mellitus group is（8.26±0.92） % the positive change of IL-6 in COPD combine with diabetes mellitus group is （20. 865=2.73）%. Compare with normal group, the positive change of IL-6 of other rise （P〈0.01）. Compare with group COPD, the positive change of IL-6 of group diabetes mellitus is lower （P〈0.01）, while the positive change of group COPD and diabetes mellitus is higher （P〈0.01）. The positive change of IL-6 of group COPD combine with diabetes mellitus is higher than that of group diabetes mellitus. Conclusion The positive change of IL-6 is increased in rats was associated with COPD inflammation and inflammatory effects of hyperglycemia correlated overlap.