依达拉奉对急性百草枯中毒致大鼠肺损伤的保护作用

Protective effect of edaravone on lung injury caused by acute paraquat poisoning in rats

目的探讨依达拉奉(edaravone,ED)对急性百草枯(paraquat,PQ)中毒致肺损伤的作用及可能机制。方法 108只SPF级SD大鼠随机分为空白对照组、PQ组和治疗组。PQ和治疗组灌胃(40mg/kg)染毒建立中毒模型,对照组等量生理盐水灌胃。染毒后30 min治疗组腹腔注射ED(4mg/kg),1次/d。检测染毒后12、24、48、72h血清超氧化物歧化酶(SOD)、丙二醛(MDA)含量及第14天、第28天血清转化生长因子β1(TGF-β1)和Ⅲ型前胶原肽(PⅢP)水平,同时观察肺组织病理改变。结果与对照组比较,PQ和治疗组血清SOD活力明显下降,MDA含量、TGF-β1和PⅢP水平均明显升高,尤以PQ组为甚,差异有统计学意义(P<0.05)。随染毒时间延长,PQ和治疗组肺组织相继发生肺泡炎和肺纤维化,但治疗组病理改变较轻。结论 ED可抑制脂质过氧化反应,调控细胞因子表达和释放,对PQ引起的肺损伤具有一定保护作用。

Objective To explore the effect of edaravone on the lung injury induced by acute paraquat （PQ） poisoning and its mechanism. Methods A total of 108 SPF level Sprague-Dawley （SD） rats were randomly divided into control group, poisoned group and edaravone treated group. Rats in poisoned group and treated group were administered intraperitoneally with 40 mg/kg PQ, control rats were only given normal saline ; the rats of treated group were intraperitoneally injected with 4 mg/kg edaravone （ED） 30 minutes after PQ injection. Then the SOD activity and MDA content in serum of rats were measured at 12, 24, 48 and 72 h and the levels of TGF-β1 and PIIIP were measured at 14 and 28d after PQ exposure, respectively. Results The results showed that compared with control group, the serum SOD activity significantly decreased, and MDA, TGF-β1 and PIIIP obviously increased in poisoned group and edaravone treated group at all the time points, especially the poisoned group （P 〈 0. 05）. The lung pathological examination in both PQ poisoned or ED treated rats arised the changes of pulmonary alveolitis and pulmonary fibrosis in succession, but it seemed a little slight in ED treated rats. Conclusion The results showed that ED has some protective effect on lung injury caused by PQ, the mechanism may relate to the inhibition of the lipid peroxidation, and regulate expression and release of cytokines.