宁痫颗粒对耐药性癫痫大鼠c-fos基因表达的影响

Effect of Ningxian Particles on Expression of C-fos Gene of Drug-resistant Epilepsy Rats

目的:观察宁痫颗粒联合卡马西平对耐药性癫痫大鼠即早基因c-fos表达的影响,并探讨其作用机制。方法:Wistar雄性大鼠42只,按体质量随机分为假手术组、模型组、卡马西平+宁痫颗粒高[(0.03+4.80)g.kg-1.d-1]、中[(0.03+2.40)g.kg-1.d-1]、低[(0.03+1.20)g.kg-1.d-1]剂量组、宁痫颗粒组(2.40 g.kg-1.d-1)和卡马西平组(0.03 g.kg-1.d-1),每组6只。采用海人酸海马CA3局部注射制作大鼠癫痫模型,术后第2天予苯妥英钠药物筛选14 d后制作耐药性癫痫模型。各组灌胃2周后免疫组织化学法检测大鼠即早基因c-fos表达的影响。结果:免疫组化实验显示:各治疗组大鼠耐药性癫痫模型颞叶皮层、海马CA3区c-fos阳性细胞较假手术组表达增强,密集分布,染色较深,同时又低于模型组;宁痫颗粒联合卡马西平各组均抑制c-fos蛋白的表达,作用优于卡马西平组(P<0.05)。各组间比较无显著差异性,宁痫颗粒组与卡马西平组无统计学差异。结论:宁痫颗粒联合卡马西平能抑制即早基因c-fos表达,两者联合疗效明显增强,以联合宁痫颗粒高剂量组疗效最佳,但治疗组指标均完全恢复到假手术组水平。

Objective ： To observe the effect of Ningxian particles combined with Carbamazepine （ CBZ ） on immediate early gene c-los expression of drug-resistant epilepsy in rats, and to explore its mechanism. Methods ： 42 male Wistar rats were randomly divided into sham group, model group, Ningxian particles combined with CBZ high[ （0.03＋4.80） g · kg-1 · d-1], medium[ （ 0.03＋2.40 ） g · kg-1 · d-1]and low dose [ （0.03＋1.20） g · kg-1 · d-1]groups, Ningxian particles group （ 2.40 g · kg-1 · d-1 ） and CBZ group （ 0.03 g · kg-1 · d-1） （ n=6 ）. Epilepsy model in rats was induced by injection of kainic acid hippocampal CA3, PHT drug was used to screen for 14 days after the production of drug-resistant epilepsy model. Immunohistochemical method in each group was given two weeks after the detection of the immediate early gene c-fos expression in rats. Results ： Immunohisto-chemistry experiments ： resistant epilepsy model temporal cortex of rats in each treatment group, increased expression of c-fos positive ceils in hippocampal CA3 region compared to the sham group, was densely distributed, deeply stained, while lower than that of the model group ; Ningxian particles combined with CBZ inhibited the expression of c-fos protein and was superior to CBZ group （P〈0.05）. No significant differences among groups, Ningxian particles group with CBZ group had no significant difference. Conclusion ： Ningxian particles combined with CBZ can inhibit the immediate early gene c-fos expression significantly and enhance efficacy, the treatment groups indicators are fully restored to the sham-operated group level.