“全鱼”Mx基因重组构建与转导分析

Construction and Transference of “all-fish” Mx Gene Recombinant

通过分子重组将鲫Mx1基因的编码序列克隆到鲤β肌动蛋白基因启动子下游,构建成能在鱼体内表达的转"全鱼"Mx基因的重组分子。采用显微注射方法,将含有鲤β肌动蛋白基因启动子和鲫Mx1基因序列的重组片段转入草鱼受精卵中,经人工孵化和养殖获得转全鱼Mx基因草鱼。经PCR和Southern杂交检测分析,证实重组分子成功整合到2月龄转基因草鱼基因组中,为草鱼抗病育种研究奠定了基础。

The coding sequence of Mx1 gene of Carassius auratus was cloned under the control of carp β-actin gene promoter by molecular restructuring,then an all-fish Mx gene recombinant was constructed.The recombinant gene was transferred into fertilized zygotes of grass carps by micro-injection.A group of all-fish transgenic grass carps were obtained.Polymerase chain reaction（PCR） technology and Southern blot were carried out on treated grass carps fry（two months）.It verified that all-fish Mx gene had integrated into the genome of grass carps.It setted the stage for disease-resisted breeding for grass carps.