胶原/透明质酸共混体系的相容性及相互作用研究

Research on the compatibility and interaction of collagen/hyaluronic acid blend system

稀溶液粘度法研究表明,在纯水中,只有当透明质酸含量为10%时,共混物才相容;而氯化钠的加入,屏蔽了正电性的胶原分子与负电性的透明质酸分子之间的静电吸引力,从而避免了聚离子复合物形成,增强了胶原/透明质酸共混体系的相容性,使两者在任意比例都能相容。根据傅立叶红外光谱数据进行主成分分析研究表明,在纯水体系中,相容时胶原酰胺Ⅰ、Ⅱ、Ⅲ带与透明质酸的C—O—C、CO、N—H及C—OH之间形成氢键;而加入氯化钠后,只在胶原酰胺Ⅰ、Ⅱ带与透明质酸的CO、N—H及C—OH之间形成氢键。

Dilute solution viscosity showed that the collagen/hyaluronic acid blends were compatible when the content of hyaluronic acid is 10% in pure water. However, NaC1 screened the electrostatic attraction between electropositive collagen molecules and electronegative hyaluronic acid molecules. It was great to avoid forming polyionic complexes and strengthen the compatibility of blend system so that collagen and hyaluronic acid were compatible in any mass ratio. According to fourier transform infrared spectrum data, principal component analysis indicated that, in pure water, new hydrogen bonds formed between amide Ⅰ , Ⅱ and Ⅲof collagen and C- O-C, C =O, N-H, C-OH of hyaluronic acid when they were compatible. After NaCl was added, new hydrogen bonds only formed between amide Ⅰ and Ⅱ of collagen and C=O,N-H,C-OH of hyaluronic acid.