某部医院口腔科门诊空气微生物污染状况的调查

Survey on microorganism contamination in air of dental clinic in a hospital

目的了解口腔科门诊空气中的微生物分布,以便更好地指导临床消毒工作,预防医院感染。方法对某部医院口腔科门诊环境中空气污染细菌与真菌的含量进行检测,选择门诊高峰期后30 min,采用平板沉降法采集口腔科的空气样本,普通营养琼脂平板培养后计数菌落、革兰染色及分离鉴定进行判断分析。结果口腔科空气中污染微生物以细菌为主,空气中细菌总数符合国家卫生标准。革兰染色结果表明,口腔科门诊室空气中细菌大多数为革兰阳性菌,占细菌总数的83.26%。口腔科门诊空气中的真菌含量较多。其中牙周科含菌量最高,达525个/m3空气。致病菌分离出3株葡萄球菌和2株乙型溶血性链菌。结论口腔科门诊患者多,流动性大,各种急慢性感染性疾病的患者均在一般患者中间就诊,因此,提示需要进一步加强对口腔科门诊环境的消毒,以控制医院感染的发生及流行。

[ Objective] To understand the distribution of microorganisms in air of dental clinic, provide the basis for guiding the clinical disinfection and preventing the nosocomial infection. [ Methods] The contents of bacteria and fungi in air of dental clinic in a hospital were detected. The air samples were collected by the flat sedimentation method at 30 minutes after visiting peak. After the ordinary nutrient agar plate culture, the colony counting, Gram~ staining, isolation and identification were performed for analy- sis. [ Results] The main microorganism of air pollution in dental clinic was bacteria, and the total bacteria count in air met the na- tional health standards. The Gram＇s staining showed that the bacteria in air of dental clinic mainly were Gram-positive bacteria, ac- counting for 83.26% of total bacteria count. The content of fungi in air of dental clinic was high. The content of microorganism in department of periodontology was the highest, which roached 525/m3. 3 strains of Staphylococcus and 2 strains of Beta hemolytic streptococcus were isolated. [ Conclusion] There are many patients in the dental clinic with high mobility, and patients with acute and chronic infectious disease visit the clinic with general patients. Therefore, it is suggested to further strengthen the environment disinfection of dental clinic, to control the nosocomial infection.