摘要
目的:建立以高效液相色谱-串联质谱(HPLC-MS/MS)法测定血浆中舒巴坦钠浓度的方法。方法:以丹参素钠为内标,采用Agilent Zorbax SB C18(100 mm×3.0 mm,3.5μm)进行分离,通过串联质谱仪,以选择反应监测方式进行测定。用于定量分析的离子对分别为m/z 231→140(舒巴坦钠)和197→135(丹参素钠)。结果:舒巴坦钠血药浓度在0.103～51.300 mg.L-1范围内线性关系良好(r2=0.999 9),定量下限为0.103 mg.L-1,相对回收率均大于90.0%,日内和日间RSD均小于5%。结论:本方法快速、简便、准确、灵敏,适用于舒巴坦钠的人体药动学研究。
Objective ： To establish an HPLC-MS/MS method for the determination of sulbactam sodium in plasma. Method： Sodi-um danshensu as the internal standard, the plasma samples were separated on an Agilent Zorbax SB C18 column （100 mm×3.0 mm,3.5μm）. A tandem mass spectrometer equipped with ESI was used as the detector and operated in the negative ion mode. Selected re-action monitoring （SRM） using the precursor product ion combinations of m/z 231→140 and 197→135 was used to determine sulbac-tam and the internal standard, respectively. Result： The established method was able to determine sulbactam sodium in human plasma over the range of 0. 103-51. 300 mg·L^-1（r^2 = 0. 999 9）with the relative recovery from 91.62% to 102.97%. Intra-and inter-day RSDs were less than 5%. Conclusion： The method developed in the study is rapid, accurate and sensitive, and suitable for the phar- macokinetics study of sulbactam.
出处
《中国药师》
CAS
2013年第4期521-524,共4页
China Pharmacist
