三种梅毒螺旋体IgM抗体检测方法在献血员筛查中的意义评价

Evaluation of Three Kinds of Treponema Pallidum IgM Antibody Detection Method in Part the Significance of Blood Screening

目的:评价三种梅毒螺旋体IgM抗体检测方法在献血员筛查中的意义。方法:收集经过TPPA实验确诊为梅毒感染的献血员血浆标本150例,用胶体金、ELISA、WESTEN-BLOT方法进行TP-IgM检测。结果:一期梅毒、二期梅毒、潜伏梅毒的TP-IgM抗体阳性率,ELISA检测高于胶体金5%,胶体金、ELISA检测均高于WEST-EN-BLOT 5～10%。三种检测TP-IgM方法在不同时期梅毒组中,检测阳性率差异有统计学意义(P<0.05)。结论:不同检测方法对梅毒螺旋体IgM抗体检测的阳性率存在差异,快速检测胶体金法适用于快速初筛,ELISA检测适合于最终确诊。

Objective：To evaluate the significance of three Treponema pallidum IgM antibody detective assays among blood donors. Methods：One hundred and fifty blood donors who were diagnosed as syphilis Infectors by TPPA were recruited and tested by Colloidal Gold, ELISA, and Western - blot for detection of TP - IgM. Results： The positive rate of ELISA in detection of TP - IgM was 5% higher than that of Colloidal Gold, and was 10% higher than that of Western - blot among different stages of Syphilis infection. The differences were significant （ P 〈 0. 05 ）. Conclusions： There were differences among the positive rates of different assays. Rapid Colloidal Gold assay can be used as a screening test, whereas ELISA can be used as a standardize test.