B族维生素在雄性小鼠高尿酸血症中的作用

Effects of vitamins B on mice hyperuricemia

目的研究B族维生素对高尿酸血症小鼠血尿酸水平的影响及其对高尿酸血症引起的内皮功能紊乱的作用。方法 SPF级昆明雄性小鼠63只,按体质量完全随机分成6组,A、B、C、D、E和F组。A组蒸馏水处理作为对照组,B组酵母膏+乙胺丁醇混合液灌胃处理建立小鼠高尿酸血症模型作为对照组;其余4组除建立模型外,还需在C组小鼠灌胃液中加入别嘌呤醇片,在D、E和F组中分别加入低、中、高剂量的叶酸+维生素B6+维生素B12混合溶液。3周后取血测定小鼠血尿酸水平及血清一氧化氮值。结果 3周后,6组小鼠的血清尿酸水平差异有统计学意义(F=14.7469,P<0.05)。与B组比较,D、E和F组小鼠的血清尿酸水平均能在一定程度上有所降低,且差异有统计学意义[D组(217.38±74.99)μmol/L,E组(228.19±65.25)μmol/L,F组(174.48±34.60)μmol/L比B组(302.93±56.99)μmol/L,均为P<0.05]。其中F组降低更显著,但其降低效果均低于C组[C组(105.52±49.32)μmol/L比F组(174.48±34.60)μmol/L,P<0.05]。6组小鼠的血清一氧化氮水平差异有统计学意义(F=7.0499,P<0.05)。与B组比较,E组和F组的血清一氧化氮水平均升高[E组(16.52±10.95)μmol/L,F组(18.63±10.77)μmol/L比B组(2.51±3.89)μmol/L,P<0.05],且与C组比较,F组升高更明显[C组(9.35±5.65)μmol/L比F组(18.63±10.77)μmol/L,P<0.05]。血清一氧化氮水平与尿酸水平呈负直线相关(r=-0.278,P<0.05)。结论叶酸、维生素B12及维生素B6可呈剂量依赖性降低高尿酸血症小鼠的血尿酸水平;同时较大剂量的B族维生素能够在一定程度上升高血清一氧化氮水平。

Objective We constructed the hyperuricemia mice models, observed the role of vitamins B in mode,in order to find the influence of lowering uric acid and NO. Methods The 63 SPF- class male Swiss mice were randomly divided into 6 groups, A group（ control）, B group, C group, D group, E group, F group. Added mixed solution of Yeast extract ＋ Ethambutol for B, C, D, E, F groups to construct the mice models, and at the same time group C were added with AlloPurinol tablets, D group, E Group and F group were added with folic acid ＋ VB6 ＋ VB12 mixed solution for low-dose, mid-dose and high-dose separately. For 3 weeks, all mice weighed, blood sampling in mice eyes, measured serum uric acid and serum NO. Results The mice serum uric acid level was statistically difference in six groups （ F = 14.7469, P 〈0.05）. The serum uric acid level of group D, E,F were decreased compared with group B [ （217.38 ± 74. 99） μmol/L in D group, （228.19 ±65.25） μmol/L in E group, （ 174. 48 ±34. 60） μmol/L in F group vs. （ 105.52 ±49. 32） μmol/L in C group, P 〈0. 05] , and F group declined more obvious, but all of them less than C group. The mice serum NO level was statistically difference in six groups （ F = 7. 0499, P 〈 0. 05 ）. The serum NO level in E group and F group were significantly higher than in B group [ （16. 52 ±10. 95） μmol/L in E group, （ 18.63 ±10. 77） μmo]/L in F group vs. （2. 51±3.89） μmol/L in B group, P 〈 0. 05 ]. Compared with group C, F group could effectively elevate [ （18. 63 ±10. 77） μmol/L in F group vs. （9. 35 ±5.65 ） μmol/L in C group, P 〈 0. 05 ]. The serum NO level was negative linear correlation with the serum uric acid level （ r = - 0. 278, P 〈 0. 05 ）. Conclusions Vitamin groups can effectively reduce serum uric acid levels. Larger dose vitamin group is likely to reverse the endothelial cells dysfunction by increased serum levels of NO.