全氟辛烷磺酸对蚯蚓体内4种酶活性的影响

Activity of Antioxidant Enzymes of PFOS Pollution on Eisenia foetida

采用人工土壤法,研究了不同暴露浓度的全氟辛烷磺酸(PFOS)对赤子爱胜蚓(Eisenia fetida)体内过氧化氢酶(CAT)、超氧化物歧化酶(SOD)及谷胱甘肽过氧化物酶(GSH-PX)活性及蚯蚓细胞色素P450(CYP450)含量的影响。研究结果表明,各指标敏感性总体为CYP450>CAT>GSH-PX>SOD。人工土壤试验中PFOS诱导CYP450含量增加,同时又抑制CAT活性。试验第4天时,50mg/kg处理组CAT活性是对照组的2.83倍,SOD活性达到190.769 U/mg,是对照组的2.19倍;12.5 mg/kg处理组GSH-PX活性最高,CYP450含量是对照组的3.85倍。PFOS暴露浓度与蚯蚓CAT、SOD和CYP450活性存在剂量-效应关系,在PFOS环境污染诊断时,可将第4天时的CAT、GSH-PX活性和CYP450含量作为生物标志物。为赤子爱胜蚓体内抗氧化酶和CYP450作为PFOS污染的早期诊断和生态风险监测生物标志物的可行性提供科学依据。

Impact of different exposure concentrations of perfluorooctane sulfonic acid （PFOS） to the enzyme activity of CAT, SOD, GSH-PX and CYP450 in Eisenia foetida by the method of artificial soil was studied. Results showed that the sensitivity of indicators could be ranked in order to most to least effective as CYP450, CAT, GSH-PX and SOD. Artificial soil test of PFOS induced the increase in CYP450 activity and restricted the CAT activity, indicating the biological response between PFOS exposure concentrations and earthworm CYP450, CAT, SOD. Under the conditions of the test at the 4 th day and 50 mg/kg treatment group, the activity of CAT was 2.83 times that of the control group, and activity of SOD reached 190.769 U/mgprot,2.19 times that of the control group. In 12.5 mg/kg treatment group, the activity of GSH-PX was the highest, and content of CYP450 was 3.85 times that of the control group. The exposure concentration of PFOS and activity of CAT and SOD have dose-response relationship, at section 4d the activity of CAT, GSH-PX and content of CYP450 can be used as a biomarker in PFOS environmental pollution diagnosis, which would provide a scientific basis for the feasibility of Eisenia foetida antioxidase and CYP450 as early diagnosis and ecological risk monitoring biological markers.