吹扫捕集-气相色谱/质谱法分析卷烟烟丝的嗅香成分

Analysis of Olfactory Aroma Components in Cut Tobacco with Purge and Trap-Gas Chromatography-Mass Spectrometry

为了模拟卷烟的自然嗅香,改造了吹扫捕集加热装置,考察了吹扫捕集和色谱分离条件对测定结果的影响,建立了快速分析卷烟烟丝嗅香成分的吹扫捕集-气相色谱-质谱联用(P&T-GC-MS)法。在接近室温条件下,用载气吹扫烟丝,挥发物经捕集、干吹、解吸后用GC-MS定性、定量分析。采用所建方法测定了参比烤烟和国内10个牌号卷烟烟丝的嗅香成分。结果表明:①采用DB-WAXER色谱柱和Tenax捕集阱,40℃吹扫60 min,干吹1 min,180℃解吸0.5 min的实验结果最佳。②检测到170种参比烤烟烟丝嗅香成分,其中,147种的相对标准偏差(RSD)≤10%。③采用标准加入法考察的20种嗅香成分的校正曲线线性关系良好(r2>0.99)。④与静态顶空(HS)和固相微萃取(SPME)相比,该方法定性嗅香成分种类更多,定量更准确。⑤该方法具有操作简单、快速,样品提取非破坏性,灵敏度高,重复性好等优点。该方法适合各类卷烟烟丝嗅香成分的分析。

A Purge and Trap-Gas Chromatography-Mass Spectrometry （P＆T-GC-MS） method was developed for rapidly analyzing the olfactory aroma components in cut tobacco of cigarettes. The cut tobacco sample was purged with carrier gas at a condition close to room temperature; the volatiles were trapped by dry blowing and desorbed then analyzed qualitatively and quantitatively by GC-MS. In order to simulate the natural olfactory aroma of cigarette, the heating unit of P＆T was modified and the conditions for P＆T and GC-MS analysis were optimized. The olfactory aroma components in cut tobacco of domestic Virginia type reference cigarette and 10 domestic cigarette brands were tested with the developed method. The results showed that： 1） The optimum conditions were using the DB-WAXER column and Tenax trap, purging for 60 minutes at 40 ~C, dry-blowing for 1 minute, desorption for 0.5 minute at 180 ℃. 2） A total of 170 components were identified from the reference cigarette, among them, the relative standard deviations （RSDs） of 147 components were no more than 10%. 3） The 20 components investigated by standard addition showed good linear relationship （r2〉0.99）. 4） Comparing with static-headspace （HS） and solid phase micro-extraction （SPME）, this method was able to identify more aroma compounds and offer more accurate results. 5） This method was fast and simple and featured the advantages of nondestructive extraction, good repeatability and high sensitivity, it was suitable for analyzing the olfactory aroma components in cut tobacco of all kinds of cigarettes.