IP-10前炎症因子和纳米脂质体槲皮素对角质形成细胞的效应

Effect of IP-10 and nanoliposomal quercetin to cultured human keratinocytes

目的探讨IP-10及干扰素-γ（IFN-1）在银屑病发病中的机制，对比纳米脂质体槲皮素（nQ）及地塞米松（Dex）对银屑病样角质形成细胞的效应。方法将培养的HaCaT细胞分为①nQ组（40、50、60μmol／L）、②Dex组（10^-7、10^-6、10^-5moL／L）、③IFN-1组（50、100、150U／ml）、④IP-10组（20、30、40ng／μl）以及⑤不加药的对照组（C）。应用MTT实验确定各药物对HaCaT细胞的生长率（GR）及生长抑制率（GSR）。应用IP-10和c-myc的DNA／RNA斑点原位杂交技术对比检测各组RNA及DNA的杂交信号。结果各组的GR／GSR均呈剂量依赖性，nQ组的GSR高于Dex组，差异有统计学意义（P〈0．01）。原位杂交结果显示：IFN-1及IP-10的杂交信号增强，nQ及Dex的杂交信号减低，差异有统计学意义（P〈0．01）。结论IFN-1及IP-10促进角质形成细胞的生长，nQ及Dex抑制其生长，nQ的作用且优于Dex。

Objective To investigate the roles of IP-10 and interferon-γin the pathogenesis of psoria- sis and compare the effects of quercetin and dexamethasone on psoriasis like HaCaT cells. Methods Cultured HaCaT cells were divided into 5 groups ： ①nQ group （ 40,50,60 μmol/L）, ②Dex group （ 10 -7, 10-6,10-s mol/L）,③IFN- γ group（50,100,150 U/ml）,④ IP-10 group（20,30,40 ng/μl） and ⑤control group（C）. The growth rate（GR） or growth suppression rate（GSR） of nQ, Dex, IFN-γ and IP-10 were determined by MTT assay. DNA and RNA of IP-10 and c-myc hybrid signals were detected by in situ hybridization. Results The GR/GSR of each group showed a dose-dependent manner, and the GSR of nQ was higher than Dex, the difference was significant（ P 〈0. 01 ）. In situ hybridization showed that the hybrid signal was up-regulated in IFN-γ group and IP-10 group, while down-regulated in nQ group and Dex group, the differences were （ P 〈 0.01 ）. Conclusions significant Certain concentrations of interferon-γ and IP-IO can promote proliferation of HaCaT cells, while quercetin and dexamathasone can inhibit proliferation HaCaT cells. Besides, quercetin