期刊文献+

高血压大鼠血管平滑肌细胞GRP78和caspase-12表达的变化及依那普利的干预作用 被引量:5

Expression of GRP78 and caspase-12 in aortic vascular smooth muscle cells of hypertension rats and effects of enalapril on vascular remodeling
下载PDF
导出
摘要 目的:观察高血压大鼠动脉血管平滑肌细胞(VSMCs)内质网应激(ERS)相关因子葡萄糖调节蛋白78(GRP78)和半胱氨酸门冬氨酸特异性蛋白酶12(caspase-12)表达的变化,并探讨高血压时VSMCs中ERS的分子机制,以及依那普利(enalapril,Ena)对血管重构的影响。方法:将40只健康雄性SD大鼠随机分为4组,即假手术(sham)组、sham+Ena组,腹主动脉缩窄术(AAB)组及AAB+Ena组,每组10只。4周后,通过颈动脉插管法测定大鼠血压和利用图像分析系统测量大鼠主动脉中层的厚度,并通过免疫组化染色法检测GRP78和caspase-12的表达。结果:①AAB组大鼠的平均动脉压(MAP)显著高于sham组、sham+Ena组和AAB+Ena组(P<0.05)。②AAB组大鼠血管平滑肌中层厚度显著大于sham组、sham+Ena组和AAB+Ena组(P<0.05)。③AAB组大鼠GRP78和caspase-12的表达量明显高于sham组、sham+Ena组和AAB+Ena组大鼠。结论:AAB所致的高血压可触发动脉血管平滑肌细胞过长过强的ERS。Ena可能通过下调ERS的水平,逆转高血压所致VSMCs损伤的作用,对VSMCs具有保护作用。其作用机制可能与其降低caspase-12介导的ERS反应有关。 AIM: To examine the effect of endoplasmic reticulum stress (ERS) on vascular remodeling induced by hypertension and to observe the effects of enalapril on the expressions of the correlation factors GRP78 and caspase-12 in vascular smooth muscle cells (VSMCs) and their relationship with vascular changes. METHODS: Forty adult male SD rats were randomly divided into sham operation group, sham + Ena group, AAB group and the AAB + Ena group. Four weeks later, MBP was measured through carotid artery incubation, the aortic media thickness was measured by image analyses software and the expressions of GRP78 and caspase-12 in the vascular smooth muscle cells were examined by immunohisto- chemistry. RESULTS : MAP of AAB group rats was significantly higher than those of rats in sham opera- tion group, sham + Ena group and AAB + Ena group (P 〈 0. 05 ). The aortic media thickness of AAB group rats was significantly thicker than those of rats in sham operation group, sham + Ena group and the AAB + EJaa group (P 〈 0. 05). The expressions of GRF78 and caspase-12 of AAB group rats were significantly higher than those of rats in sham group, sham + Ena group and AAB + Ena group. CONCLUSION: High blood pressure resulting from abdominal aortic bounding causes endoplasmic reticulum stress response of vascular smooth muscle cells. Enalapril may exert some protective effects on VSMCs by inhibiting the endoplasmic reticulum stress via downregulation of the expression of caspase-12.
出处 《心脏杂志》 CAS 2013年第2期163-167,共5页 Chinese Heart Journal
关键词 高血压 血管平滑肌细胞 GRP78 CASPASE-12 依那普利 大鼠 hypertension vascular smooth muscle cell GRP78 caspase-12 enalapril rats
  • 相关文献

参考文献15

二级参考文献48

共引文献39

同被引文献54

  • 1刘唐威,王秋雁,伍伟锋.内质网伴侣蛋白在小鼠病毒性心肌炎心肌细胞的表达及其与细胞凋亡的关系[J].广西医科大学学报,2005,22(2):157-159. 被引量:9
  • 2娄利霞,唐朝枢.内质网应激与心血管疾病[J].中国分子心脏病学杂志,2005,5(2):496-499. 被引量:19
  • 3汪国海,霍勇,王梦德,邢厚恂,刘平,戴成祥,徐希平.中国六城市轻中度高血压患者血清叶酸水平的调查[J].卫生研究,2007,36(3):305-307. 被引量:30
  • 4姜雅秋,宋光熠,谭岩,张建军,李屹,张广新,王实.高同型半胱氨酸血症致动脉粥样硬化的实验研究[J].中国老年学杂志,2007,27(22):2169-2170. 被引量:13
  • 5Graham IM, Daly LE, Refsum HM, et al. Plasma homocysteine as a rick factor for vascular disease: The European Concerted Action Pro- jeet[J]. JAMA, 1997, 27(4) :1775 - 1781.
  • 6Sen U, Moshal KS, Tyagi N, et al. Homocysteine-induced myofibro- blast differentiation in mouse aortic endothelial cells [ J ]. J Cell Physiol, 2006, 209 ( 3 ) :767 - 774.
  • 7Yilmaz N, Ciqek HK, Celik A, et al. Diagnostic value of homocys- teine, C-reactive protein and bilirubin for coronary artery disease [J]. East Mediterr Health J, 2007, 13(3) :522 -535.
  • 8Sacco RL, Adams R, Albers G, et al. Guidelines for prevention of stroke in patients with ischemic stroke or transient ischemic attack : A statement for healthcare professionals from the American Heart Asso- ciation/American Stroke Association Council on Stroke: Co-spon- sored by the Council on Cardiovascular Radiology and Intervention: the American Academy of Neurology affirms the value of this guide- line[Jl. Stroke, 2006, 37(2):577-617.
  • 9Baumbach GL, Sigmund CD, Bottiglieri T, et al. Structure of cere- bral arterioles in cystathionine beta-synthase-deficient mice[ J]. Circ Res, 2002, 91(10):931 -937.
  • 10Tyagi SC. Homocysteine redox receptor and regulation of extracellular matrix components in vascular cells [ J ]. Am J Physiol, 1998, 274 ( 2 Pt 1 ) : C396 - Cd05.

引证文献5

二级引证文献28

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部