膜联蛋白A5在喉癌组织中的表达及临床意义

Expression of annexin A5 in human laryngeal carcinoma and its clinical significance

目的通过观察膜联蛋白A5(annexin A5,ANXA5)在喉癌组织中的表达,探讨ANXA5在喉癌中的作用。方法采用免疫组化SP法及RT-PCR法检测53例不同临床分期、病理学分级及颈部淋巴结转移的喉癌组织与25例癌旁组织标本中ANXA5及其基因的表达情况。结果喉癌组织中ANXA5水平高于癌旁组织中ANXA5的水平(P<0.0 5);ANXA5的表达与临床分期、病理学分级及颈部淋巴结转移密切相关(P<0.05),与患者年龄、性别及临床分型无关(P>0.05)。结论 ANXA5可能在喉癌的发生、发展中发挥重要作用。

Objective To study the role of annexin A5 （ANXA5） in laryngeal carcinoma via detecting the expression of ANXA5 in human laryngeal carcinoma tissue. Methods Protein and gene expression of ANXA5 was detected with immunohistochemical technique and RT-PCR. 53 specimens of laryngeal carcinoma and 25 of adjacent normal tissues from laryngeal carcinoma patients with various clinical stages and pathologic classification. Results Over-expression of gene and protein of ANXA5 was observed in human laryngeal carcinoma tissues （ P 〈 0.05 ） , and the expression levels of ANXA5 were closely associated with the clinical stages, pathologic classification and cervical lymphatic metastasis of laryngeal carcinoma （ P 〈 0. 05 ） and irrelevant with patients ＇ age, gender and clinical classification （ P 〉 0.05 ）. Conclusions ANXA5 may play an important role in the occurrence and development of human laryngeal carcinoma.