小鼠输卵管、子宫和卵巢组织中的TGF-αmRNA活性

Activity of TGF α mRNA in the Mouse Oviduct,Uterus and Ovary

采用 RT-PCR技术分别检测了排卵期和怀孕 1～ 5d小鼠输卵管、子宫及卵巢组织中的转化生长因子-α( TGF-α) m RNA活性。结果发现 ,这一期间内小鼠输卵管、子宫及卵巢中均有较强的 TGF-α m RNA表达 ,其中输卵管和卵巢中的表达量变化不大 ,而子宫中的表达量在怀孕 5d时明显减少。这进一步证实外源 (旁分泌 )

This study was conducted to examine the activity of TGF α mRNA by RT RCR technique in the mouse oviduct,uterus and ovary at the time of ovulation and during days 1 5 of pregnancy.A very strong activity of TGF α mRNA was observed in the oviduct,the uterus and the ovary of the mouse during the whole period studied.The levels of expression in the oviduct and the ovary did not change markedly,but the level of expression declined in the uterus on day 5 of pregnancy.These results suggested that TGF α,in the way of paracrine,may play a key role in mouse early embryo development.