miR-196b mRNA和HoxB8 mRNA在结直肠癌组织中的表达及其临床意义

Expressions and Significance of miR-196b mRNA and HoxB8 mRNA in Human Colorectal Cancer

目的研究miR-196b和HoxB8在结直肠癌组织中的表达及与其临床病理特征的关系,并探讨在结直肠癌组织中miR-196b与靶基因HoxB8表达的关系。方法用实时荧光定量PCR技术检测30例结直肠癌组织及对应的正常黏膜组织中miR-196b mRNA和HoxB8 mRNA的表达量,用Western blot法检测HoxB8蛋白的表达。结果 miR-196b mRNA和HoxB8 mRNA在结直肠癌组织中的表达量均高于其在对应的正常黏膜组织中的表达量(P<0.05)。miR-196b mRNA的表达与结直肠癌的淋巴结转移、肿瘤分期(Ⅰ+Ⅱ与Ⅲ+Ⅳ)及远处转移均有关(P<0.05),而与肿瘤部位、大小、大体类型、浸润深度、组织分化程度、患者的年龄及性别均无关(P>0.05);HoxB8mRNA的表达与结直肠癌的上述临床病理特征均无关(P>0.05)。miR-196b mRNA与HoxB8 mRNA的表达存在负相关(r=-0.458,P<0.05),而与HoxB8蛋白的表达无明显相关性(r=-0.236,P>0.05)。结论 miR-196bmRNA及HoxB8 mRNA在结直肠癌组织中均呈高表达,miR-196b mRNA的高表达与结直肠癌的发生、发展及预后有关。miR-196b可能通过与靶基因HoxB8 mRNA的3′非编码区结合抑制HoxB8 mRNA的表达。

Objective To research the expressions ofmiR-196b and HoxB8 mRNA in colorectal cancer and their correlation with clinicopathologic features, and to explore the relationship between miR-196b and HoxB8 in vivo. Methods Expressions of RNA （including miR-196b and HoxB8 mRNA） and HoxB8 protein were detected respectively by using quantitative real-time reverse transcriptase PCR and Western blot in 30 cases of colorectal cancer and corresponding normal mucous membrane tissues. Results In colorectal cancer tissues, expressions ofmiR-196b and HoxB8 mRNA were higher than those of the corresponding normal mucous membrane tissues （.P〈 0.05）. Expression of miR 196b mRNA was assoc- iated with lymph node metastasis, neoplasm stages （ Ⅰ ＋ Ⅱ and Ⅲ ＋ Ⅳ ）, and distant metastasis （P〈 0. 05）, on the other hand, no significant differences were observed regarding tumor site, size, gross type, depth of invasion, tissue differentiation, age, and sex （P〉0. 05） . Expression ofHoxB8 mRNA was no significant differences concerning lymph node metastasis, tumor stages （ Ⅰ＋ Ⅱ, Ⅲ ＋Ⅳ ）, distant metastasis, tumor site, size, gross type, depth of invasion, tissue differentiation, age, and sex （P〉 0. 05）. The expression of miR-196b mRNA was negatively correlated with HoxB8 mRNA expression （r=-0. 458, P〈 0. 05）, and HoxB8 protein expression with no obvious correlation （r=-0. 236, P〉 0. 05） in colorectal cancer tissues. Conclusions The expressions of miR-196b and HoxB8 mRNA in colorectal cancer tissues are higher, the high expression of miR-196b mRNA is related to the tumorigenesis and progression of colorectal cancer as well as correlated with prognosis in colorectal cancer. The miR-196b inhibits the expression of HoxB8 mRNA by binding to the 3＇-UTR of target HoxB8 mRNA.