发光菌法在线监测水中生物毒性

Online monitor of biological toxicity in water by luminescent bacteria method

随着人类工农业活动的增加,水体污染物种类及毒性作用日益复杂化。对水中生物毒性的在线监测,已经迫在眉睫。根据发光菌法快速、灵敏、低廉特点,将发光菌法运用于水中在线生物毒性监测,能客观地反映水中有毒有害物质的毒性,实时反映水中毒性的变化。本方法使用明亮发光杆菌作为测试菌种,标准品为10 mg/L的7H2O.Zn-SO4,最佳测试温度在15℃,菌种复苏后稳定时间为24 h,通过测定实际水样,加标抑制率为95.94～97.02%。

With the increase of human activities in industry and agriculture, the species and the toxic effect of water pollutant are getting more and more complex. Therefore, the online monitor of the aquatic toxicity has been imminent. Advantaged in rapidness, sensitivity and cheapness, the luminescent bacteria method can be applied into the online monitor, which can objectively reflect the toxicity of toxic substances as well as the change of it. Photobacterium phosphoreum is used as the test strains in this method, and the standard is 10 mg/L 7H2O . ZnSO4, the best test temperature is 15℃ and the strain recovery time stabilization time after recovery is 24 hours. The proposed method was successfully applied to measure actual water sample, proforma rate of addition standard is between 95.94% and 97.02%.