大剂量岷当归对大鼠脑缺血再灌注损伤MPO表达的影响

Experimental study on effect of high-dose Angelica sinensis on myeloperoxidase expression in rats with cerebral ischemia/reperfusion injury

目的:探讨大剂量岷当归对大鼠脑缺血再灌注后神经损伤的保护作用及脑髓过氧化物酶(MPO)的影响。方法:将Wistar大鼠100只随机分为假手术组,模型组,当归低、中、高剂量组,将除假手术组外的其余组大鼠制成大脑中动脉缺血再灌注模型,当归低、中、高剂量组分别在恢复血流后给予不同剂量的当归灌胃治疗,比较治疗前后大鼠神经缺损症状评分并检测MPO含量的变化。结果:治疗前模型组和当归低、中、高剂量组大鼠神经缺损症状评分均显著高于假手术组(P<0.01),治疗后评分,当归低、中、高剂量组与模型组比较,均有降低(P<0.01),当归低、中剂量组与高剂量组比较,评分较高(P<0.01);各组大鼠脑组织MPO活性比较,假手术组MPO活性最低,模型组脑组织中MPO活性明显升高,各治疗组同模型组相比均降低,差异有统计学意义(P<0.01),且低剂量组高于中、高剂量组,差异有统计学意义(P<0.05,P<0.01)。结论:岷当归对大鼠脑缺血再灌注后神经损伤具有保护作用,且当归的用量越大,对损伤后神经的保护作用也越大。

Objective ：To investigate the protective effect of high -dose Angelica sinensis on nerve injury and its impact on the myeloper- oxidase （MPO） expression in brain tissue among rats with cerebral ischemia/reperfusion （I/R） injury. Methods：A total of 100 Wistar rats were randomly divided into sham operation group,model group,and low- dose, middle -dose,and high -dose Angelica sinensis groups. A rat model of middle cerebral artery I/R was induced in all groups except the sham operation group. The low -dose ,middle -dose ,and high -dose Angelica sinensis groups received Angelica sinensis by gavage after reperfusion~ The neurological deficit score and MPO content were meas- ured before and after treatment. Results： Before treatment, the model group and high - dose, middle - dose, and low - dose Angelica sinensis groups had significantly higher neurological deficit scores than the sham operation group （P 〈0. 01 ）. After treatment ,the neurological deficit scores decreased significantly in the high -dose,middle -dose,and low -dose Angelica sinensis groups and model group （P 〈0. 01 ） ,and the low - dose and middle - dose Angelica sinensis groups had significantly higher neurological deficit scores than the high - dose Angelica sinen- sis group （ P 〈0. 01 ）. The MPO activity in brain tissue of rats in each group were compared to results ,the sham operation group had the lowest MPO activity;the MPO activity increased significantly in the model group;the high- dose,middle- dose,and low- dose Angelica sinensis groups had significantly lower MPO activity than the model group （P 〈0. 01 ）,and the MPO activity was significantly higher in the low- dose Angelica sinensis group than in the middle -dose and high -dose Angelica sinensis groups （P 〈0. 05 or P 〈0. 01 ）. Conclusion ：Angelica sineusis has pro- tective effect on nerve injury after cerebral I/R in rats ,and the protective effect improves as the dose of Angelica sinensis increases.