一株产海因酶菌种的筛选与鉴定

Screening and Identification of a Strain of Hydantoinase-producing Microorganism

通过对土壤中微生物以单一海因类似物作为唯一氮源富集、分离和纯化,利用双层琼脂法筛选获得产海因酶菌株,以基因工程手段提取其DNA,并进行特异性的16S rDNA测序,最终利用Blastn工具将其基因序列与GenBank数据库中的进行序列比对。研究表明:该菌与Bacillus megateriumQM B1551(NC004604)序列相似性达到98%,证明该菌为巨大芽孢杆菌。

Through gathering,isolation and purification ,a strain of Hydantoinase-producing microorganisms from soil microbial based on single hydantoin analogue was taken as the onlynitrogen source. The Hydantoinase-producing microorganisms was screened by utilizing the Double agar-agar method. Then,it DNA was extracted by the means of genetic engineering method,and sequence of its specificity 16S rD- NA was tested. Lastly,a comparison its gene sequence to GenBank database by Blastn tool. Research re- sult shows ：this microorganism has 98 % similarity with Bacillus megaterium QM B1551 （NC _ 004604）, which proved that it is Bacillus megaterium.