纳米炭吸附氟尿嘧啶对胃癌组织及转移淋巴结细胞凋亡的影响

Effect of 5-fluorouracil-loaded carbon nanoparticles on apoptosis of cells in gastric carcinoma and lymph node metastasis

目的探讨纳米炭吸附氟尿嘧啶淋巴靶向化疗对胃癌转移淋巴结、胃癌组织及正常胃组织中细胞凋亡率(apoptosis index,AI)的影响。方法我院36例胃癌患者分为淋巴靶向化疗(lymph node-targeted chemotherapy,LNTC)组和对照组,每组18例。LNTC组先以纳米炭吸附氟尿嘧啶行淋巴靶向化疗后再行手术,对照组直接手术。术毕即取胃癌组织、转移淋巴结及正常胃组织,以Tunel-AP法检测其中的细胞凋亡率情况。结果 LNTC组胃癌组织及转移淋巴结中AI为(17.30±3.50)%及(32.64±4.72)%,对照组胃癌组织及转移淋巴结中AI为(4.81±2.76)%及(7.03±2.15)%。LNTC组胃癌组织及转移淋巴结中AI均高于对照组,两组间差异有统计学意义(P<0.05).LNTC组正常胃组织中AI为(13.73±3.60)%,对照组正常胃组织中AI为(14.10±3.87)%,两组间AI无明显差异(P>0.05)。结论纳米炭吸附氟尿嘧啶能靶向作用于胃癌组织及转移淋巴结,使其凋亡率升高,促进肿瘤细胞死亡,而对正常胃组织的细胞凋亡无明显影响。

Objective To investigate the efficacy of 5 -fluorouracil -loaded carbon nanoparticles, the targeted chemothera- peutic drug, on apoptosis index of cells in metastatic lymph nodes, gastric cancer and the normal gastric tissues. Methods Thirty - six patients with gastric cancer in this department were divided into lymph node - targeted chemotherapy group （ n = 18 ） and control group （ n = 18 ）. The chemotherapy group had operation after the lymphatic target chemotherapy with 5 - fluorouracil - loaded carbon nanoparticles. However, in control group, the patients were operated directly. The apoptosis index of cells in gastric carcinoma tissues, metastatic lymph nodes, and the normal gastric tissues after the operation were detected by TUNEL. Results In the lymph node - tar- geted chemotherapy group, apoptosis index（AI） of cells in gastric carcinoma tissues and metastatic lymph nodes were （17.30±3.50） % and （ 32.64±4.72） %, respectively, however, in the control group these were （ 4.81±2.76 ） % and （ 7.03±2.15 ） %, respectively. Consequently, the apoptosis index of cells in gastric carcinoma tissues and metastatic lymph nodes in the lymph node - targeted chemotherapy group was higher than that in the control group （ P 〈 0.05 ）. Meanwhile, in the lymph node - targeted chemo- therapy group, the AI of cells in normal gastric tissues was （13.73±3.60）% ; however in the control group, the apoptosis index of cells in nomal gastric tissues was （ 14.10±3.87）% . There was no significant difference in the apoptosis index between the two groups （P 〉0.05 ）. Conclusions 5 -Fluorouraeil- loaded carbon nanoparticles can target gastric carcinoma tissues and metastatic lymph nodes, therefore, increasing the apoptosis index which can promote tumor cell death. However, it has no obvious effect on the apopto- sis index in normal gastric tissues.