骨碎补总黄酮对胶原诱导大鼠类风湿关节炎骨破坏治疗作用的实验研究

Therapeutic effect of drynaria total flavonoids on the bone destruction of CIA rat

目的建立模拟类风湿关节炎(RA)发病的胶原诱导关节炎(CIA)大鼠模型,明确骨碎补总黄酮对CIA大鼠关节局部骨破坏的改善作用,为临床提供实验依据。方法利用皮下注射牛Ⅱ型胶原诱导Wistar大鼠发病,建立CIA大鼠模型。将大鼠随机分为正常对照组(10只)、灭菌用水治疗组(10只)、骨碎补总黄酮治疗组(10只)。每只大鼠均在炎症分值达到2分及以上时开始治疗,分别在治疗4周和8周,取胫骨上段做硬组织切片,观察胫骨上段骨小梁变化及骨量变化情况。结果骨碎补总黄酮具有明显抑制CIA大鼠关节周围骨量丢失的作用,且其抑制骨量减少主要是通过抑制骨小梁数量减少来实现的,与抑制骨小梁宽度丢失关系较小。结论骨碎补总黄酮能明显抑制CIA大鼠关节侵蚀及关节周围骨丢失。

Objective ： Through model, we aimed to confirm the potential experimental evidence for clinical usage. the establishment of the collagen induced arthritis （CIA） rat curative effect of drynaria total flavonoids （DTF） to supply Methods Subcutaneous injection of bovine type II collagen was used to induce Wistar rats to fall ill, and then the CIA rat model was establised. The rats are randomly divided into normal control group （n = 10）, sterilized water treatment group （n = 10） and DTF treatment group （n = 10）. Every rat began to receive treatment while inflammation score reached two points and above. After 4-week and 8 - week treatment, we killed the rats, took the left femur and fifth lumbar vertebrae to have bone biomechanics check, scanned proximal tibia by micro-CT, made hard-tissue slices, and then observed the changes in bone biomechanical with femur and lumbar spine of each group, and evaluated trabecular variation and bone quantity changes of proximal tibia of each group. Results The DTF group had obvious inhibition on periarticular bone loss of CIA rat and the a- bility mainly depended on the inhibition on the loss of trabecular bone number, not trabecular width loss. Conclusion DTF has obvious inhibition on joint erosion and periarticular bone loss of CIA rat.