摘要
目的观察瑞舒伐他汀(ROS)对心肌梗死后大鼠Periostin表达的影响,探讨其改善心肌重构的机制。方法雌性SD大鼠30只,结扎左前降支建立急性心肌梗死(AMI)模型,术后24h随机分为AMI组和ROS组各15只;另设15只为假手术组(Sham组),只穿线不结扎。ROS组大鼠每天给予ROS 10mg/kg(溶于2mL清水)灌胃,另两组每天以2mL清水灌胃。4周后比较各组大鼠胶原容积分数(CVF)、Periostin蛋白及转化生长因子-β1(TGF-β1)和Periostin的mRNA表达差异。结果与AMI组相比,ROS组心肌CVF、Periostin蛋白及TGF-β1和Periostin的mRNA均显著降低(P<0.05),ROS组和AMI组上述指标均显著高于Sham组(P<0.05)。结论 ROS能够降低受损心肌中Periostin表达水平,可能是其改善心肌重塑的机制之一。
Objective To evaluate the effects of rosuvastatin(ROS) on expression of Periostin protein in rats with acute myocardial infarction and to investigate the improvement of myocardial reconstruction mechanism.Methods Thirty female SD rats with ligated LAD were used to establish models of acute myocardial infarction(AMI),twenty four hours later,the models were randomly divided into acute myocardial infarction(AMI) as control group(n=15) and ROS group(n=15);Another 15 rats were tested with pierced but not ligated LAD(n=15) as sham-operated group(Sham group).Rats in ROS group were given ROS by gavage at 10 mg/kg dose levels to soluble in 2 mL clear water,whereas rats in the other two groups were given 2 mL clear water each day.Four weeks later,compare the differences of collagen volume fraction(CVF),Periostin protein,TGF-β1 mRNA and Periostin mRNA.Results Myocardial CVF,Periostin protein,TGF-β1 mRNA and Periostin mRNA were reduced in the ROS group compared with rats in the AMI group(P<0.05),both ROS group and AMI group were significantly higher than those in the Sham group(P<0.05).Conclusion ROS can reduce the expression of Periostin in the damaged myocardial,may be one of the mechanism of myocardial remodeling improvement.
出处
《重庆医学》
CAS
CSCD
北大核心
2013年第11期1204-1206,共3页
Chongqing medicine
