环氧合酶-2蛋白在胃癌组织中的表达

Expression of Cyclooxygenase-2 Protein in Human Gastric Cancer

背景：流行病学调查发现长期使用非甾体类抗炎药（NSAIDs）的患者结肠癌发病率明已下降。NSAIDs能抑制环氧合酶（COX），而COX-2在结肠癌的发生、发展中起一定作用。目的：调查COX-2蛋白在胃癌组织中的表达。方法：用免疫组化半定量方法检测COX－2和COX-1蛋白在胃癌及相应正常组织中的表达。结果：97．1％（33／34）的胃癌组织中有COX-2蛋白表达，其强度明显高于正常胃窦粘膜，分化型胃癌的表达强度明显高于差分化型，进展期胃癌和有淋巴结转移者的表达亦增强；胃体腺、肠化上皮和间质细胞中可见COX-2蛋白表达。COX-1蛋白在正常胃窦粘膜、胃癌和癌旁组织的间质细胞中显低强度表达。结论：COX-2可能在胃癌的发生、分化和浸润转移中起作用。

Background/Aims: Epidemiological studies have shown that chronic intake of nonsteroidal antiinflammatory drugs (NSAIDs) can inhibit cyclooxygenase (COX) activity and reduce the incidence of human colon cancer. The inducible COX-2 may play a ro1e in colon carcinogenesis. The purpose of this study was to investigate the expression of COX-2 protein in human gastric cancer. Methods: COX-2 and COX-1 protein expression were examined by immunohistochemistry in gastric cancer and its paired substantial normal tissue. Results: Increased expression of COX-2 protein was present in the cytoplasmic region of cancer cells in gastric adenocarcinomas (33/34) as compared with that of normal con trol group. There was a positive correlation between tumor histology or metastasis and COX-2 protein expression. COX-2 was also observed in some interstitial cells, metaplastic glandular cells and nearly all fundic glandular cells. COX-1 protein expression was present in some interstitial cells but rarely seen in the epithelial cells. Conclusions: COX-2 may play a role in human gastric carcinogenesis.