黄曲霉毒素解毒酶制剂对饲喂黄曲霉毒素B1饲粮的断奶仔猪生长性能及肝脏生化指标的影响

Effects of Aflatoxin-Detoxifizyme on Growth Performance and Hepatic Biochemical Indexes of Weaner Piglets Fed Diets Containing Aflatoxin B_1

本试验旨在通过研究黄曲霉毒素解毒酶制剂(aflatoxin-detoxifizyme,ADTZ)对饲喂黄曲霉毒素B1(AFB1)饲粮的断奶仔猪生长性能及肝脏生化指标的影响,探讨其应用效果。选用日龄相差不超过3 d、品种相同的断奶仔猪108头,按照遗传背景相同、体重相近、性别比例一致的原则随机分为3个组,分别为对照组(基础饲粮)、AFB1组(基础饲粮+0.1 mg/kg AFB1)、ADTZ组(基础饲粮+0.1 mg/kg AFB1+0.2%ADTZ),每组6个重复,每个重复6头仔猪。试验期30d。结果表明:1)与对照组相比,AFB1组仔猪平均日增重、平均日采食量有下降趋势(P>0.05),料重比有上升趋势(P>0.05);肝脏中谷胱甘肽还原酶、过氧化氢酶、琥珀酸脱氢酶活性显著下降(P<0.05),超氧化物歧化酶、谷胱甘肽过氧化物酶与胆碱酯酶活性下降,但差异不显著(P>0.05),碱性磷酸酶活性和丙二醛含量有上升趋势(P>0.05);2)与对照组相比,当添加0.2%的ADTZ后,仔猪平均日增重、平均日采食量均有所改善,同时有降低料重比的趋势(P>0.05);以上相关生化指标均恢复到正常水平。因此,在基础饲粮中添加AFB1可导致断奶仔猪生长性能下降,肝脏生理功能受损;添加ADTZ可有效消除AFB1对断奶仔猪的危害,改善其生长性能,保护肝脏生理功能。

This experiment was conducted to evaluate the effects of aflatoxin-detoxifizyme （ADTZ） on the growth performance and hepatic biochemical indexes of weaner piglets fed diets containing aflatoxin B1 （AFB1）, and investigated the application effect. A total of 108 piglets, whose age difference was not more than three days, and with the same genetic background, body weight, and consistent sex ratio, were randomly divided into 3 groups： control group （basal diet）, AFB1 group （basal diet＋0.1 mg/kg AFB1） and ADTZ group （basal diet＋0.1 mg/kg AFB1＋0.2% ADTZ）, and with 6 replicates per group. The experiment lasted for 30 days. The results showed as follows： 1） compared with control group, in AFB1 group, average daily gain （ADG） and average daily feed intake （ADFI） were decreased （P〉0.05）, and feed/gain was increased （P〉0.05）; the activities of glutathione reductase, catalase and succinic dehydrogenase were significantly decreased （P〈0.05）, and the activities of superoxide dismutase, glutathione peroxidase and choline esterase were decreased （P〉0.05）, while alkaline phosphatase activity and MDA content were increased （P〉0.05）; 2） compared with control group, in ADTZ group, ADG and ADFI were increased （P〉0.05）, and feed/gain was increased （P〉0.05）; biochemical indexes were returned to normal levels. Adding AFB1 to the basal diet can lead to a decline in growth performance and hepatic physiological functions; adding ADTZ can effectively reduce the harm of AFB1 on weaner piglets, improve growth performance and protect the hepatic physiological functions.