红参含药血清对人乳腺癌MCF-7细胞增殖和细胞周期的影响

Effect of Serum Containing Radix Ginseng Rubra on Cell Proliferation and Cell Cycle in Human Breast Cancer Cell Line MCF-7

[目的]研究红参含药血清对人乳腺癌MCF-7细胞增殖和细胞周期的影响。[方法]采用MTT法观察红参含药血清对人乳腺癌细胞MCF-7的增殖活性。流式细胞术检测人乳腺癌细胞MCF-7的细胞周期、细胞凋亡率。[结果]红参低剂量含药血清对MCF-7细胞有促增殖作用,24、48、72h时增殖率分别为105.9%、115.9%和121.5%。而高剂量对MCF-7细胞表现为抑制作用。红参低剂量组使MCF-7细胞S期DNA含量比例明显增多,高剂量组使细胞周期阻滞在G0/G1期,并能够诱导细胞凋亡,凋亡率达26.86%。[结论]红参含药血清在高剂量时抑制MCF-7细胞生长,低剂量时对细胞具有促增殖作用。

[Purpose] To investigate the effect of serum containing Radix Ginseng Rubra on cell proliferation and cell cycle in human breast cancer cell line MCF-7. [Methods] The cell prnliferation of serum containing different concentrations of Chinese medicine＂Radix Ginseng Rubra＂ on human breast cancer cell line MCF-7 in vitro were detected by MTT＂ method, and cell cycle of human breast cancer cell line MCF-7 were examined by flow eytometry.[Results] Serum containing Radix Ginseng Rubra with lower con- centration couht make a proliferative effect in MCF-7 cell with proliferation rate 105.9%, 115.9%, 121.5%, at 24,48,72h respectively,but serum containing Radix Ginseng Rubra with higher concentration inhibited the growth of MCF-7 cells;serum containing Radix Ginseng Rubra with lower concentratinn could increase DNA percentage in S phase of cell cycle. Serum containing Radix Ginseng Rubra with higher concentration could increase the number of cells in G0/G1 phase and induce cell apoptosis with apoptosis rule of 26.86%. [ Conchlsion] Serum containing Radix Ginseng Rubra with lower concentration could make a prulii＇erative effeel in MCF-7 cell,and which with higher concentration enuld inhibit the grnwlh and induce cell apoptosis in MCF-7 cell.