大豆下胚轴不定芽对卡那霉素和氯化钠耐性的研究

Kanamycin and NaCl Tolerance of Adventitious Shoot Induced from Hypocotyl in Soybean

为探讨不同基因型大豆对卡那霉素和NaCl的耐性,以大豆黑农44、合丰35和淮豆9号的下胚轴为外植体,分别研究了不同浓度的卡那霉素和NaCl对不同基因型大豆下胚轴不定芽形成的影响。结果表明:大豆不同基因型对卡那霉素和NaCl的耐受性不同,且在伸长培养42 d时随卡那霉素和NaCl浓度的增加,不定芽诱导率、芽数和芽长均显著降低;黑农44、合丰35和淮豆9号卡那霉素的筛选浓度分别为100,150,100 mg·L-1,NaCl的筛选浓度分别为100,100,75 mmol·L-1。

In order to discuss the tolerance of different genotypes of soybean varieties to kanamycin and NaC1, hypocotyl from soybean genotypes including Heinong 44,Hefeng 35 and Huaidou 9 were used as the explants to investigate the formation of adventitious shoot under stress with kanamycin and NaCI. The results showed that the tolerance to kanamycin and NaC1 varied with genotypes involved. With the increasing of kanamycin and NaC1 concentration, the induction rate of adventitious shoot, number of shoot and length of shoot were significantly decreased in three genotypes. The critical concentration of kanamycin for Heinong 44,Hefeng 35 and Huaidou 9 were 100,150 and 100 mg·L-1 and that of NaC1 for three genotypes were 100,100 and 75 mmol· L -1, respectively.