旋毛虫感染对过敏性哮喘小鼠血清及肺泡灌洗液总IgE的影响

Effect of Trichinella spiralis infection on total IgE levels in surum and bronchoalveolar lavage fluid from mice with allergic asthma

【目的】通过研究旋毛虫感染小鼠在过敏性哮喘发病时血清及肺泡灌洗液中总IgE水平变化,探讨旋毛虫感染对过敏性哮喘的影响。【方法】取雌性、8周龄BALB/c小鼠,随机分为3组,A组为空白对照组,B组为单纯过敏性哮喘组,C组为感染旋毛虫后哮喘组。C组经灌胃感染旋毛虫囊包幼虫200～300条;4周后,以卵清白蛋白(ovalbumin,OVA)分别对B组和C组小鼠进行致敏激发,建立过敏性哮喘模型;8周后,取小鼠血清、肺泡灌洗液,检测总IgE水平。【结果】ELISA法检测血清中A、B、C组小鼠总IgE水平分别为(61.79±25.79)、(437.08±75.68)、(251.64±107.27)ng/ml。与B组相比较,C组小鼠总IgE水平降低(P<0.05);肺泡灌洗液中A、B、C三组小鼠总IgE水平分别为(43.70±29.49)、(387.49±148.32)、(102.50±49.55)ng/ml。与B组相比较,C组小鼠总IgE水平降低(P<0.05)。【结论】旋毛虫感染具有降低过敏性哮喘小鼠总IgE水平的作用。

[Objective] To investigate the effect of Trichinella spiralis infection on allergic asthma, through the study of the change of total IgE levels in surum and bronchoalveolar lavage fluid （BALF） when the mice which were infected with Trichinella spiralis were suffering from allergic asthma. [ Methods ] Female BALB/c mice were randomly divided into three groups （six mice each）, including A as control group, B as allergic asthma group, C as asthma followed by Tdchinella spiralis infection. The animals in group C were intragastrically infected with 200-300 Trichinella spiralis muscle larvae. Four weeks later, ovalbumin （OVA） was used to induce allergic asthma for mice in group B and C, in order to establish experimental animal model. After eight weeks, all mice were killed and total IgE levels were measured in serum and BALF. [ Results ] Total IgE levels were measured by ELISA. Total IgE levels from serum in group A, B and C were （61.79 ± 25.79）, （437.08 ± 75.68）, （251.64 ± 107.27） ng/ml. Total IgE level in group C was lower than that in group B （P〈 0.05）. BALF total IgE levels in group A, B and C were （43.70 ± 29.49）, （387.49 ± 148.32）, （102.50 ± 49.55） ng/ml. Total IgE level in group C was lower than that in group B （P 〈 0.05）. [ Conclusion ] Trichinella spiralis infection can inhibit the total IgE level of mice with the allergic asthma.