毛苕子籽多糖的微波提取工艺优选及抗氧化活性考察

Optimization of Microwave Extraction Technology and Investigation of Antioxidant Activity for Polysaccharides from Seeds of Vicia villosa

目的:优选毛苕子籽多糖的微波提取工艺并考察其初步抗氧化活性。方法:以多糖提取量为指标,采用L9(34)正交试验考察微波温度、微波时间、料液比和pH对毛苕子籽多糖提取工艺的影响,确定最佳提取条件。利用苯酚硫酸法测定多糖含量,Fention反应检测毛苕子籽多糖的抗氧化活性。结果:毛苕子籽多糖的最佳提取条件为微波温度50℃,微波处理5min,pH 7,料液比1∶25,多糖提取量30.043 mg.g-1;抗氧化性试验表明毛苕子籽多糖具有较强的抗氧化活性。结论:优选的提取工艺稳定可行,为毛苕子籽多糖的综合利用开发提供实验依据。

Objective：To optimize microwave extraction technology of polysaccharides from seeds of Vicia villosa,and preliminarily investigate its antioxidant activity.Method：With extraction amount of polysaccharides as index,L9（34） orthogonal test was adopted to optimize extraction technology of polysaccharides from seeds of V.villosa with liquid-solid ratio,pH,microwave temperature and time as factors.The content of polysaccharides was determined by phenol sulfuric acid method,antioxidant activity of polysaccharides from seeds of V.villosa was detected by Fention reaction.Result：Optimum extraction conditions for polysaccharides from seeds of V.villosa were as followings：microwave extraction temperature 50 ℃,microwave treatment time 5 min,solid-liquid ratio 1∶25 with pH 7;Under these conditions,extraction amount of polysaccharides was 30.043 mg·g-1.Antioxidant activity test showed that polysaccharides from seeds of V.villosa had strong antioxidant activity.Conclusion：This optimized technology was stable and feasible,it could provide experimental basis for comprehensive utilization and development of seeds of V.villosa.