摘要
目的:优选板蓝根中总生物碱的超声提取工艺。方法:以总生物碱提取量为指标,采用L9(34)正交试验考察乙醇体积分数、超声时间,料液比对提取工艺的影响,确定最佳提取工艺。结果:各因素对超声提取工艺的影响顺序为乙醇体积分数>超声时间>料液比;最佳提取工艺为乙醇体积分数80%,提取时间20 min,料液比1∶10。结论:优选的提取工艺稳定可行,提取率高。
Objective：To optimize ultrasonic extraction process of total alkaloids in Isatis indigotica.Method：With extraction amount of total alkaloids as index,effects of ethanol concentration,liquid-solid ratio,ultrasonic time on extraction technology was investigated by by L9（34） orthogonal test.Result：The influencing order of each factors on ultrasonic-assistant extraction process of total alkaloids was ethanol concentration＆gt;extraction time＆gt;liquid-solid ratio.Optimum extraction process was as following：extracted 20 min with 10 times the amount of 80% ethanol.Conclusion：This optimized technology was stable and feasible with high extraction rate.
出处
《中国实验方剂学杂志》
CAS
北大核心
2013年第9期66-68,共3页
Chinese Journal of Experimental Traditional Medical Formulae
关键词
板蓝根
总生物碱
正交试验
提取工艺
Isatis indigotica
total alkaloids
orthogonal design
extraction process
