摘要
目的:建立瓜蒌饮片中总糖及还原糖的含量测定方法,并进行测定和比较。方法:采用苯酚-硫酸法测定多糖的含量,采用3,5-二硝基水杨酸法(DNS法)测定还原糖的含量。结果:全国15个省市的18批市售瓜蒌饮片中,总糖的含量介于16.17%～32.65%,还原糖含量介于3.29%～13.29%。结论:建立的瓜蒌饮片中总糖和还原糖的测定方法简便、准确;优质瓜蒌饮片中的总糖含量>25%,还原糖含量>9%;总糖和还原糖含量的高低可以诠释瓜蒌"糖味浓者为佳"的传统观点。
Objective：To establish a method for determination of total sugar and reducing sugar in processed Fructus Trichosanthis,and to detect and compare the content of sugars with the methods.Method： The content of total sugar and reducing sugar was determined by phenol-sulfuric acid method and 3,5-dinitrosalicylic acid（DNS） method,respectively.Result： The content of total sugar and reducing sugar was 16.17%-32.65% and 3.29%-13.29%,respectively,in 18 batches of processed Fructus Trichosanthis purchased from 15 provinces.Conclusion： These methods are easily operated and reliable.The content of total sugar and reducing sugar in processed Fructus Trichosanthis with good quality is above 25% and 9%,respectively.The content of total sugar and reducing sugar can explain the traditional opinion that Fructus Trichosanthis with higher sugar content is of better quality.
出处
《中国实验方剂学杂志》
CAS
北大核心
2013年第9期96-99,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
2011年中医药行业科研专项(201107009)
关键词
瓜蒌
总糖
还原糖
苯酚-硫酸法
3
5-二硝基水杨酸法
栝楼
Fructus Trichosanthis
total sugar
reducing sugar
phenol-sulfuric acid method
3,5-dinitrosalicylic acid method
Trichosanthes kirilowii
