PCR-DGGE法研究新型氧肥对水淹胁迫下土壤细菌多样性的影响

Effects of New Oxygen Fertilizer on Soil Bacterial Diversity Under Waterlogging by PCR-DGGE Method

以短埂大参土壤为研究对象,以水淹为胁迫因子,将短埂大参土壤进行A:单一水淹处理,B:水淹施加新型氧肥处理,C:正常浇水不施加新型氧肥处理,处理时间为36 d。对各处理第6、12、18、24、30、36天土壤中细菌16S rDNA基因的PCR扩增产物进行DGGE分析。结果表明,同时期的A处理与B处理样品的DGGE条带相似度随处理时间延长,相似度降低,B处理的第12、18、24天样品的DGGE条带数比A多且较亮;B处理的香农-威纳指数(Shannon指数)比A处理的Shannon指数高,差值呈"低-高-低"的趋势;同时期的B处理与C处理样品DGGE条带相似度较低;各处理在各检测时间内多样性表现不同,A样品土壤细菌多样性在各检测时间的相似度普遍较低,B样品土壤细菌多样性在各检测时间的相似度较高且较稳定,C样品土壤细菌多样性在各检测时间的相似度较低。说明新型氧肥对水淹胁迫下土壤细菌多样性影响较显著。

We used Macropanax rosthornii soil as research subject, and water as the stress factor. The experiment was tested 36 days under the following three different conditions ： A just was waterlogged ; B was waterlogged with new oxygen fertilizer; C was normally watered without new oxygen fertilizer. Amplifying 16S rDNA genes of bacteria by PCR, subsequently the products of PCR were analyzed by DGGE on the sixth, twelfth, eighteenth, twenty-fourth, thirtieth and thirty-sixth day. The results indicate that at the same stage, with the prolongation of processing time, condition A and condition B＇s DGGE bands similarity are reduced ; on the twelfth, eighteenth and twenty fourth day, the DGGE bands of condition B are more and brighter than condition A. The Shannon index of B is higher than A, and the difference presents the trend of ＂low-high- low＂. The DGGE bands of B and C owns lower similarity during the same period. The diversity of different conditions presents differently during the detection time. Under the condition A, the similarity of soil bacteria diversity is generally lower at each detection time. Under the condition B, the similarity of soil bacteria diversity is higher and stable at each detection time. Under the condition C, the similarity of soil bacteria diversity is lower at each detection time. The research reveals the new oxygen fertilizer has a significant effect on waterlogged soil bacterial diversity.